Anti-PKC zeta (phospho T560)抗体(ab59412)
Key features and details
- Rabbit polyclonal to PKC zeta (phospho T560)
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-PKC zeta (phospho T560)抗体
参阅全部 PKC zeta 一抗 -
描述
兔多克隆抗体to PKC zeta (phospho T560) -
宿主
Rabbit -
特异性
Detects endogenous levels of PKC? only when phosphorylated at threonine 560. -
经测试应用
适用于: WB, ELISA, IHC-Pmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human PKC zeta aa 500-600 (phospho T560).
Database link: Q05513 -
阳性对照
- WB: Extracts from COS7 cells treated with PMA (125ng/ml, 30mins). IHC-P: Human brain gliomas, human brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59412于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
|
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| ELISA |
1/20000.
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| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
|
ELISA
1/20000. |
|
IHC-P
1/50 - 1/100. |
靶标
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功能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
组织特异性
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
结构域
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
翻译后修饰
CDH5 is required for its phosphorylation at Thr-410. -
细胞定位
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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数据库链接
- Entrez Gene: 5590 Human
- Omim: 176982 Human
- SwissProt: Q05513 Human
- Unigene: 496255 Human
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别名
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
图片
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Western blot - Anti-PKC zeta (phospho T560) antibody (ab59412)All lanes : Anti-PKC zeta (phospho T560) antibody (ab59412) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with PMA (125ng/ml, 30mins)
Lane 2 : Extracts from COS7 cells treated
with PMA (125ng/ml, 30mins) with phosphopeptide
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody (ab59412)Immunohistochemical analysis of paraffin embedded human brain gliomas using ab59412 at 1/50 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta (phospho T560) antibody (ab59412)Immunohistochemical analysis of paraffin embedded human brain tissue using ab59412 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide. -
ELISA - Anti-PKC zeta (phospho T560) antibody (ab59412)ELISA showing that ab59412 (1:20000) detects endogenous levels of PKC zeta only when phosphorylated at Thr560.
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ELISA - Anti-PKC zeta (phospho T560) antibody (ab59412)Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody, ab59412.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab59412 被引用在 12 文献中.
- Patel R et al. Simultaneous inhibition of atypical protein kinase-C and mTOR impedes bladder cancer cell progression. Int J Oncol 56:1373-1386 (2020). PubMed: 32236625
- Asim M et al. Ketamine Alleviates Fear Generalization Through GluN2B-BDNF Signaling in Mice. Neurosci Bull 36:153-164 (2020). PubMed: 31444653
- Taoro-Gonzalez L et al. Extracellular cGMP Reverses Altered Membrane Expression of AMPA Receptors in Hippocampus of Hyperammonemic Rats: Underlying Mechanisms. Mol Neurobiol 56:4428-4439 (2019). PubMed: 30328550
- Islam SMA et al. The modulation of actin dynamics via atypical Protein Kinase-C activated Cofilin regulates metastasis of colorectal cancer cells. Cell Adh Migr 13:106-120 (2019). PubMed: 30417717
- Taoro-Gonzalez L et al. Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms. J Neuroinflammation 15:36 (2018). WB ; Rat . PubMed: 29422059