Anti-PKC zeta抗体- C-terminal (ab226772)
Key features and details
- Rabbit polyclonal to PKC zeta - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PKC zeta抗体- C-terminal
参阅全部 PKC zeta 一抗 -
描述
兔多克隆抗体to PKC zeta - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Cow, Pig, Zebrafish
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免疫原
Synthetic peptide within Human PKC zeta (C terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q05513 -
阳性对照
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IP: HEK-293T whole cell extract.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226772于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 68 kDa.
|
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| IP |
1/100 - 1/500.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 68 kDa. |
|
IP
1/100 - 1/500. |
靶标
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功能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
组织特异性
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
结构域
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
翻译后修饰
CDH5 is required for its phosphorylation at Thr-410. -
细胞定位
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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数据库链接
- Entrez Gene: 286877 Cow
- Entrez Gene: 5590 Human
- Entrez Gene: 18762 Mouse
- Entrez Gene: 100533200 Pig
- Entrez Gene: 100009538 Rabbit
- Entrez Gene: 25522 Rat
- Entrez Gene: 555737 Zebrafish
- Omim: 176982 Human
see all -
别名
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
图片
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Western blot - Anti-PKC zeta antibody - C-terminal (ab226772)All lanes : Anti-PKC zeta antibody - C-terminal (ab226772) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 68 kDa10% SDS-PAGE gel.
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Immunoprecipitation - Anti-PKC zeta antibody - C-terminal (ab226772)PKC zeta was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract with 5 µg ab226772. Western blot was performed from the immunoprecipitate using ab226772. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Control IgG IP in HEK-293T whole cell extract.
Lane 2: ab226772 IP in HEK-293T whole cell extract.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab226772 尚未被引用在任何文献中。