Anti-PKC zeta抗体(ab59364)
Key features and details
- Rabbit polyclonal to PKC zeta
- Suitable for: ELISA, IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Xenopus laevis, African green monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-PKC zeta抗体
参阅全部 PKC zeta 一抗 -
描述
兔多克隆抗体to PKC zeta -
宿主
Rabbit -
特异性
ab59364 detects endogenous levels of total PKC zeta protein. The immunogen used for this product shares 89% homology with PKC iota/lambda. Cross-reactivity with this protein has not been confirmed experimentally. -
经测试应用
适用于: ELISA, IHC-P, IP, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human, Xenopus laevis, African green monkey -
免疫原
Synthetic peptide within Human PKC zeta aa 526-575. The exact sequence is proprietary. Synthesized non-phosphopeptide derived from human PKC zeta around the phosphorylation site of threonine 560 (Q-L-TP-P-D).
Database link: Q05513 -
阳性对照
- IHC-P: Human proliferative endometrial tissue, human brain tissue. ICC: NIH/3T3 cells. WB: Rat hippocampus lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59364于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
|
| IHC-P | (1) |
1/50 - 1/100.
|
| IP |
Use at an assay dependent concentration.
|
|
| WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.
|
| ICC/IF |
1/100 - 1/500.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-P
1/50 - 1/100. |
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 68 kDa. |
|
ICC/IF
1/100 - 1/500. |
靶标
-
功能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
组织特异性
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
结构域
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
翻译后修饰
CDH5 is required for its phosphorylation at Thr-410. -
细胞定位
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
-
数据库链接
- Entrez Gene: 5590 Human
- Entrez Gene: 18762 Mouse
- Entrez Gene: 25522 Rat
- Omim: 176982 Human
- SwissProt: Q05513 Human
- SwissProt: Q02956 Mouse
- SwissProt: P09217 Rat
- Unigene: 496255 Human
see all -
别名
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
图片
-
Western blot - Anti-PKC zeta antibody (ab59364)Verified CustomerAnti-PKC zeta antibody (ab59364) at 1/500 dilution + Xenopus laevis cytoplasmic egg extract at 40 µg
Secondary
Goat polyclonal - IRDye® 800CW at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 5 seconds
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab59364)Image from Rivero R et al., Reproductive Biology and Endocrinology 2012, 10:17, 5 March 2012. Fig 1.; doi: 10.1186/1477-7827-10-17.Immunohistochemical analysis of human proliferative endometrial tissue, staining PKC zeta with ab59364.
Antigen retrieval was performed by heat mediation in a citrate buffer. Tissue was blocked with blocking solution before incubating with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB. -
Immunocytochemistry/ Immunofluorescence - Anti-PKC zeta antibody (ab59364)Immunofluorescence analysis of NIH/3T3 cells using PKC zeta at dilution of 1:100.
-
Western blot - Anti-PKC zeta antibody (ab59364)All lanes : Anti-PKC zeta antibody (ab59364) at 1/500 dilution
Lane 1 : Extracts from COS-7 (African green monkey kidney fibroblast-like cell line) cells, treated with PMA (125 ng/ml, 30mins), without immunizing peptide
Lane 2 : Extracts from COS7 (African green monkey kidney fibroblast-like cell line)cells, treated with PMA (125 ng/ml, 30mins), plus immunizing peptide
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted? -
Western blot - Anti-PKC zeta antibody (ab59364)This image is courtesy of an Abreview submitted by Mr Richard D'MelloLanes 2-4 : Anti-PKC zeta antibody (ab59364) at 1/500 dilution
Lanes 1-2 : Rat hippocampus lysate at 40 µg
Lanes 3-4 : Rat hippocampus lysate at 20 µg
Secondary
All lanes : Anti-Rabbit IR800-linked conjugation: IRDye® 800CW
Performed under reducing conditions.
Predicted band size: 68 kDa
Exposure time: 5 minutes
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC zeta antibody (ab59364)ab59364 at a 1/50 dilution staining PKC zeta in formalin/PFA fixed paraffin-embedded human brain tissue using Immunohistochemistry.
Left panel: Without immunizing peptide.
Right image: Plus immunizing peptide.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (26)
ab59364 被引用在 26 文献中.
- Kaushik M et al. Environment Enrichment Facilitates Long-Term Memory Consolidation through Behavioral Tagging. eNeuro 10:N/A (2023). PubMed: 36941060
- Machado Batista Sohn J et al. Phosphodiesterase 4 inhibition after retrieval switches the memory fate favoring extinction instead of reconsolidation. Sci Rep 13:20384 (2023). PubMed: 37990053
- Muta Y et al. Enhanced SREBP2-driven cholesterol biosynthesis by PKCλ/ι deficiency in intestinal epithelial cells promotes aggressive serrated tumorigenesis. Nat Commun 14:8075 (2023). PubMed: 38092754
- Ma R et al. LGL1 binds to Integrin β1 and inhibits downstream signaling to promote epithelial branching in the mammary gland. Cell Rep 38:110375 (2022). PubMed: 35172155
- Liu S et al. G3BP1 regulates breast cancer cell proliferation and metastasis by modulating PKCζ. Front Genet 13:1034889 (2022). PubMed: 36330442