Anti-PKC theta/PRKCQ (phospho S676)抗体(ab47774)
Key features and details
- Rabbit polyclonal to PKC theta/PRKCQ (phospho S676)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PKC theta/PRKCQ (phospho S676)抗体
参阅全部 PKC theta/PRKCQ 一抗 -
描述
兔多克隆抗体to PKC theta/PRKCQ (phospho S676) -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PKC theta/PRKCQ aa 600-700 (C terminal) (phospho S676).
Database link: Q04759 -
阳性对照
- IHC-P: Human breast carcinoma. WB: JK cell extract treated with 200nM PMA for 30mins.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab47774于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
组织特异性
Skeletal muscle, megakaryoblastic cells and platelets. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
结构域
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻译后修饰
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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数据库链接
- Entrez Gene: 5588 Human
- Omim: 600448 Human
- SwissProt: Q04759 Human
- Unigene: 498570 Human
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别名
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774)This image shows human breast carcinoma stained with ab47774 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phosphopeptide, whereas the left hand image is untreated.
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Western blot - Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774)All lanes : Anti-PKC theta/PRKCQ (phospho S676) antibody (ab47774) at 1/500 dilution
Lane 1 : JK cell extract treated with 200nM PMA for 30mins. No peptide.
Lane 2 : JK cell extract, untreated. No peptide.
Predicted band size: 82 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab47774 被引用在 2 文献中.
- Li J et al. Nuclear PKC-? facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci 129:2448-61 (2016). WB ; Human . PubMed: 27149922
- Aguiló JI et al. Protein kinase C-theta is required for NK cell activation and in vivo control of tumor progression. J Immunol 182:1972-81 (2009). WB ; Mouse . PubMed: 19201850