重组Anti-PKC theta/PRKCQ抗体[EPR1488Y] (ab52494)
![Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)](https://www.abcam.cn/ps/products/52/ab52494/Images/ab52494-256948-anti-pkc-theta-antibody-epr1488y-western-blot.jpg "Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1488Y] to PKC theta/PRKCQ
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PKC theta/PRKCQ抗体[EPR1488Y]
参阅全部 PKC theta/PRKCQ 一抗 -
描述
兔单克隆抗体[EPR1488Y] to PKC theta/PRKCQ -
宿主
Rabbit -
特异性
This antibody was tested with PKC alpha, beta, gamma, delta, zeta, eta, iota recombinant proteins and was found to react with alpha, beta, gamma, delta, eta proteins.
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经测试应用
适用于: WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PKC theta/PRKCQ. The exact sequence is proprietary.
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阳性对照
- WB: Rat brain lysate, Rat spleen lysate, Mouse C2C12 cell lysate; Jurkat cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1488Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52494于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa).
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| 说明 |
|---|
|
WB
1/500. Detects a band of approximately 85 kDa (predicted molecular weight: 85 kDa). |
应用说明
Is unsuitable for ICC/IF or IHC-P.
靶标
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功能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
组织特异性
Skeletal muscle, megakaryoblastic cells and platelets. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
结构域
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻译后修饰
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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数据库链接
- Entrez Gene: 5588 Human
- Entrez Gene: 18761 Mouse
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q02111 Mouse
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
see all -
别名
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
图片
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Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)All lanes : Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified)
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : C2C12 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)](https://www.abcam.cn/ps/products/52/ab52494/Images/ab52494-256947-anti-pkc-theta-antibody-epr1488y-western-blot.jpg)
Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/1000 dilution (purified) + Jurkat whole cell lysate at 10 µg
Secondary
Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution
Predicted band size: 85 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST -
![Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)](https://www.abcam.cn/ps/products/52/ab52494/Images/ab52494-64766-anti-pkc-theta-antibody-epr1488y-western-blot.jpg)
Western blot - Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494) at 1/500 dilution (unpurified) + Jurkat cell lysate at 10 µg
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa
Observed band size: 85 kDa -
![Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)](https://www.abcam.cn/ps/products/52/ab52494/Images/ab52494-4-benefits-of-recombinant-antibodies.png)
Anti-PKC theta/PRKCQ antibody [EPR1488Y] (ab52494)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab52494 被引用在 1 文献中.
- Tao P et al. Thrombospondin-1 aggravates colonic mucosal inflammatory injuries via promoting the differentiation of CD11c+ macrophages with lysosomal activity limited in colitis. Ann Transl Med 9:1738 (2021). PubMed: 35071432