重组Anti-PKC theta/PRKCQ抗体[EPR1487(2)] (ab110728)
![Western blot - Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)](https://www.abcam.cn/ps/products/110/ab110728/Images/ab110728-124020-anti-pkc-theta-antibody-epr14872-western-blot.jpg "Western blot - Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1487(2)] to PKC theta/PRKCQ
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PKC theta/PRKCQ抗体[EPR1487(2)]
参阅全部 PKC theta/PRKCQ 一抗 -
描述
兔单克隆抗体[EPR1487(2)] to PKC theta/PRKCQ -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human platelet and Jurkat lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1487(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110728于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 82 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 82 kDa. |
应用说明
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
靶标
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功能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
组织特异性
Skeletal muscle, megakaryoblastic cells and platelets. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
结构域
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻译后修饰
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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数据库链接
- Entrez Gene: 5588 Human
- Entrez Gene: 18761 Mouse
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q02111 Mouse
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
see all -
别名
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
图片
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Western blot - Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)All lanes : Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Jurkat lysate
Lysates/proteins at 10 µg/ml per lane.
Predicted band size: 82 kDa -
![Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)](https://www.abcam.cn/ps/products/110/ab110728/Images/ab110728-2-benefits-of-recombinant-antibodies.png)
Anti-PKC theta/PRKCQ antibody [EPR1487(2)] (ab110728)
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab110728 被引用在 6 文献中.
- Chi JN et al. MAP4K3/GLK inhibits Treg differentiation by direct phosphorylating IKKβ and inducing IKKβ-mediated FoxO1 nuclear export and Foxp3 downregulation. Theranostics 12:5744-5760 (2022). PubMed: 35966593
- van Senten JR et al. Use of CRISPR/Cas9-edited HEK293 cells reveals that both conventional and novel protein kinase C isozymes are involved in mGlu5a receptor internalization. J Biol Chem 298:102466 (2022). PubMed: 36087841
- Søgaard D et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
- Byerly J et al. PRKCQ promotes oncogenic growth and anoikis resistance of a subset of triple-negative breast cancer cells. Breast Cancer Res 18:95 (2016). PubMed: 27663795
- Bracamontes CG et al. The serum protein profile of early parity which induces protection against breast cancer. Oncotarget 7:82538-82553 (2016). WB ; Human . PubMed: 27769065
- Søgaard D et al. Training Does Not Alter Muscle Ceramide and Diacylglycerol in Offsprings of Type 2 Diabetic Patients Despite Improved Insulin Sensitivity. J Diabetes Res 2016:2372741 (2016). PubMed: 27777958