Anti-PKC mu/PKD (phospho Y463)抗体(ab59415)
Key features and details
- Rabbit polyclonal to PKC mu/PKD (phospho Y463)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PKC mu/PKD (phospho Y463)抗体
参阅全部 PKC mu/PKD 一抗 -
描述
兔多克隆抗体to PKC mu/PKD (phospho Y463) -
宿主
Rabbit -
特异性
Detects endogenous levels of PKC µ/PKD only when phosphorylated at tyrosine 463 (corresponding to mouse tyrosine 469 or rat tyrosine 200).
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PKC mu/PKD aa 400-500 (phospho Y463).
Database link: Q15139 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59415于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
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| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
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IHC-P
1/50 - 1/100. |
靶标
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功能
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
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数据库链接
- Entrez Gene: 5587 Human
- Omim: 605435 Human
- SwissProt: Q15139 Human
- Unigene: 508999 Human
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别名
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
图片
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Western blot - Anti-PKC mu/PKD (phospho Y463) antibody (ab59415)All lanes : Anti-PKC mu/PKD (phospho Y463) antibody (ab59415)
Lane 1 : HepG2 cell lysates
Lane 2 : HepG2 cell lysates with Phospho peptide -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC mu/PKD (phospho Y463) antibody (ab59415)Immunohistochemical analysis of paraffin embedded human brain tissue using ab59415 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab59415 被引用在 3 文献中.
- Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). PubMed: 29153837
- Zheng H et al. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. J Biol Chem 286:35733-41 (2011). WB ; Human . PubMed: 21865166
- Arun SN et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . PubMed: 21132013