Anti-PKC mu/PKD (phospho S910)抗体(ab131267)
Key features and details
- Rabbit polyclonal to PKC mu/PKD (phospho S910)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PKC mu/PKD (phospho S910)抗体
参阅全部 PKC mu/PKD 一抗 -
描述
兔多克隆抗体to PKC mu/PKD (phospho S910) -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human PKC mu/PKD (phospho S910) conjugated to keyhole limpet haemocyanin.
Database link: Q15139 -
阳性对照
- 293 cell extract treated with EGF; HUVEC cell extract treated with PMA; HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab131267 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131267于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa).
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa). |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
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数据库链接
- Entrez Gene: 5587 Human
- Entrez Gene: 18760 Mouse
- Entrez Gene: 85421 Rat
- Omim: 605435 Human
- SwissProt: Q15139 Human
- SwissProt: Q62101 Mouse
- SwissProt: Q9WTQ1 Rat
- Unigene: 508999 Human
see all -
别名
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
图片
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All lanes : Anti-PKC mu/PKD (phospho S910) antibody (ab131267) at 1/500 dilution
Lane 1 : 293 cell extract treated with EGF
Lane 2 : 293 cell extract untreated
Lane 3 : HUVEC cell extract treated with PMA
Lane 4 : HUVEC cell extract untreated
Predicted band size: 102 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of methanol-fixed HeLa cells labelling PKC mu/PKD (phospho S910) with ab131267 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab131267 尚未被引用在任何文献中。