Anti-PKC gamma (phospho T655)抗体(ab5796)
Key features and details
- Rabbit polyclonal to PKC gamma (phospho T655)
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PKC gamma (phospho T655)抗体
参阅全部 PKC gamma 一抗 -
描述
兔多克隆抗体to PKC gamma (phospho T655) -
宿主
Rabbit -
特异性
PKC alpha (69%) and beta 2 (62%) may cross-react in cells expressing high levels of these proteins. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide corresponding to PKC gamma (phospho T655).
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阳性对照
- WB: HeLa cells treated with PMA, a phorbol ester. IHC-P: Mouse cerebellum tissue, human cerebellum tissue.
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常规说明
PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PKC gamma. The final product is generated by affinity chromatography using a PKC gamma-derived peptide that is phosphorylated at threonine 655. -
Primary antibody说明
PKC gamma is an 80 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC gamma plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication. The activation loop threonine (threonine 514 in PKC gamma) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme. Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKC gamma. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/10 - 1/50.
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| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80 kDa.
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| 说明 |
|---|
|
IHC-P
1/10 - 1/50. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 80 kDa. |
靶标
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功能
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
组织特异性
Expressed in Purkinje cells of the cerebellar cortex. -
疾病相关
Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA). -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5582 Human
- Entrez Gene: 18752 Mouse
- Entrez Gene: 24681 Rat
- Omim: 176980 Human
- SwissProt: P05129 Human
- SwissProt: P63318 Mouse
- SwissProt: P63319 Rat
- Unigene: 631564 Human
see all -
别名
- KPCG_HUMAN antibody
- MGC57564 antibody
- OTTHUMP00000067291 antibody
see all
图片
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Western blot - Anti-PKC gamma (phospho T655) antibody (ab5796)
Peptide Competition and Phosphatase treatment: Lysates prepared from HeLa cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-11) or treated with lambda (ë) phosphatase (12), blocked with a 3% low-fat milk-TBST buffer overnight at 4°C, and incubated with 0.50
µ g/mL ab5796 antibody for two hours at room temperature in a 3% BSA TBST buffer, following prior incubation with: no peptide (1, 11, 12), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine containing peptide (3), the phosphopeptide immunogen (4), or, the phosphopeptide corresponding to the immunogen from other PKC isoforms (5-10). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStarTM method. The data show that the peptide corresponding to PKC gamma [pT655] blocks the an -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)Paraffin embedded human cerebellum tissue (right) stained for PKC gamma using ab5796 at 1/20 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC gamma (phospho T655) antibody (ab5796)Paraffin embedded mouse cerebellum tissue (right) stained for PKC gamma using ab5796 at 1/100 dilution in immunohistochemical analysis. Negative control without primary antibody (left).
数据表及文件
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Datasheet download
文献 (3)
ab5796 被引用在 3 文献中.
- Zhao L et al. Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ1 signaling pathway in diabetic atherosclerosis. Mol Med 28:4 (2022). PubMed: 35062863
- Jin M et al. Prediction and verification of the AD-FTLD common pathomechanism based on dynamic molecular network analysis. Commun Biol 4:961 (2021). PubMed: 34385591
- Fujita K et al. Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology. Nat Commun 9:433 (2018). PubMed: 29382817
