Anti-PKC gamma抗体(ab4144)
Key features and details
- Rabbit polyclonal to PKC gamma
- Suitable for: ELISA, IHC-Fr, IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PKC gamma抗体
参阅全部 PKC gamma 一抗 -
描述
兔多克隆抗体to PKC gamma -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-Fr, IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mammals
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免疫原
Synthetic peptide:
FEACNYPLELYEVSRYGPSSS
, corresponding to amino acids 302-322 of Human PKC gamma. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA | ||
| IHC-Fr | ||
| IHC-P | ||
| ICC/IF | (1) | |
| WB | (1) |
| 说明 |
|---|
应用说明
ELISA: assay dependent.
Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for immunoprecipitation of PKC gamma.
Immunohistochemistry: 1/250 (using horseradish peroxidase or alkaline phosphatase).
Western blot (using horseradish peroxidase or alkaline phosphatase): 1/250. Predicted molecular weight: 84 kDa.
Not tested in other applications.
Optimal dilutions / concentrations should be determined by the end user.
With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
It has also been reported to be useful for immunoprecipitation of PKC gamma.
靶标
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功能
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
组织特异性
Expressed in Purkinje cells of the cerebellar cortex. -
疾病相关
Defects in PRKCG are the cause of spinocerebellar ataxia type 14 (SCA14) [MIM:605361]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA14 is an autosomal dominant cerebellar ataxia (ADCA). -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 5582 Human
- Omim: 176980 Human
- SwissProt: P05129 Human
- Unigene: 631564 Human
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别名
- KPCG_HUMAN antibody
- MGC57564 antibody
- OTTHUMP00000067291 antibody
see all
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab4144 尚未被引用在任何文献中。