重组Anti-PKC epsilon抗体[EPR28194-50] (ab317498)
![Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)](https://www.abcam.cn/ps/products/317/ab317498/Images/ab317498-542617-anti-pkc-epsilon-antibody-epr28194-50-western-blot-human-hypothalamus-tissue-lysate-20-ug.jpg "Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR28194-50] to PKC epsilon
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PKC epsilon抗体[EPR28194-50]
参阅全部 PKC epsilon 一抗 -
描述
兔单克隆抗体[EPR28194-50] to PKC epsilon -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human hypothalamus tissue, Human cerebellum tissue, Human hippocampus tissue, Mouse cerebral cortex tissue, Mouse skeletal muscle tissue, Mouse heart tissue, Rat cerebral cortex tissue, Rat skeletal muscle tissue, Rat heart tissue, U-2 OS whole cell, IMR-32 whole cell, NIH/3T3 whole cell, PC-12 whole cell, HeLa whole cell, 293T whole cell, NIH/3T3 whole cell, PC-12 whole cell and His-tagged Human PKC epsilon fragment lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR28194-50 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 84 kDa.
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| 说明 |
|---|
|
WB
1/1000. Predicted molecular weight: 84 kDa. |
靶标
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功能
This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
结构域
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻译后修饰
Phosphorylation on Thr-566 by PDPK1 triggers autophosphorylation on Ser-729. - Information by UniProt
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数据库链接
- Entrez Gene: 5581 Human
- Entrez Gene: 18754 Mouse
- Entrez Gene: 29340 Rat
- Omim: 176975 Human
- SwissProt: Q02156 Human
- SwissProt: P16054 Mouse
- SwissProt: P09216 Rat
- Unigene: 580351 Human
see all -
别名
- KPCE_HUMAN antibody
- MGC125656 antibody
- MGC125657 antibody
see all
图片
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Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)All lanes : Anti-PKC epsilon antibody [EPR28194-50] (ab317498) at 1/1000 dilution
Lane 1 : Human hypothalamus tissue lysate
Lane 2 : Human cerebellum tissue lysate
Lane 3 : Human hippocampus tissue lysate
Lane 4 : Human skeletal muscle tissue lysate
Lane 5 : Human spleen tissue lysate
Lane 6 : Mouse cerebral cortex tissue lysate
Lane 7 : Mouse skeletal muscle tissue lysate
Lane 8 : Mouse heart tissue lysate
Lane 9 : Rat cerebral cortex tissue lysate
Lane 10 : Rat skeletal muscle tissue lysate
Lane 11 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-5 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Lanes 6-11 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 84 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 6 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: heart (PMID:1556149), spleen (PMID:1556149), skeletal muscle.
The identity of the bands between 25 kDa and 50 kDa are unknown.
Lanes 6-11 are applied with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 and lanes 1-5 are applied with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)](https://www.abcam.cn/ps/products/317/ab317498/Images/ab317498-542620-anti-pkc-epsilon-antibody-epr28194-50-western-blot-u-2-os-human-bone-osteosarcoma-epithelial-cell-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)All lanes : Anti-PKC epsilon antibody [EPR28194-50] (ab317498) at 1/1000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lane 2 : HEL (human erythroleukemia erythroblast) whole cell lysate
Lane 3 : HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 84 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: HEL, HepG2.
The identity of the lower MW band at approximately 26 kDa (in lanes 1-3) is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)](https://www.abcam.cn/ps/products/317/ab317498/Images/ab317498-542621-anti-pkc-epsilon-antibody-epr28194-50-western-blot-imr-32-human-neuroblastoma-neuroblast-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)All lanes : Anti-PKC epsilon antibody [EPR28194-50] (ab317498) at 1/1000 dilution
Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
Lanes 2 & 6 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Lanes 3 & 7 : PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate
Lane 4 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 5 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 84 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The identity of the lower MW band at approximately 26 kDa (in lanes 1-7) are unknown.
The identity of the higher MW band at approximately 240 kDa (only detected in fresh cells) is unknown.
In lanes 1-3, the lysate was stored at -80? prior to Western Blotting. The bands between 37 kDa and 84 kDa are likely to be degradation products. In lanes 4-7, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
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![Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)](https://www.abcam.cn/ps/products/317/ab317498/Images/ab317498-542623-anti-pkc-epsilon-antibody-epr28194-50-western-blot-his-tagged-human-pkc-epsilon-fragment-10-ng.jpg)
Western blot - Anti-PKC epsilon antibody [EPR28194-50] (ab317498)All lanes : Anti-PKC epsilon antibody [EPR28194-50] (ab317498) at 1/1000 dilution
Lane 1 : His-tagged Human PKC epsilon fragment
Lane 2 : GST-tagged full-length Human PKC alpha recombinant protein
Lane 3 : GST-tagged full-length Human PKC beta 1 recombinant protein
Lane 4 : GST-tagged full-length Human PKC beta 2 recombinant protein
Lane 5 : GST-tagged full-length Human PKC gamma recombinant protein
Lane 6 : GST-tagged full-length Human PKC delta recombinant protein
Lane 7 : GST-tagged full-length Human PKC eta recombinant protein
Lane 8 : GST-tagged full-length Human PKC theta recombinant protein
Lane 9 : GST-tagged full-length Human PKC mu recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 84 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody does not cross-react with human PKC alpha, PKC beta 1, PKC beta 2, PKC gamma, PKC delta, PKC eta, PKC theta, PKC mu.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
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![Anti-PKC epsilon antibody [EPR28194-50] (ab317498)](https://www.abcam.cn/ps/products/317/ab317498/Images/ab317498-5-benefitsofrecombinantantibodies.jpg)
Anti-PKC epsilon antibody [EPR28194-50] (ab317498)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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