Name: Anti-PKC beta 2 antibody [Y125]
Brand: RabMAb
SKU: ab32026
Rating: 4 (6 reviews)

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Flow Cytometry (Intracellular) - Anti-PKC beta 2 antibody [Y125] (ab32026)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y125] to PKC beta 2
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human, Recombinant fragment

Alexa Fluor® 647 Carrier Free HRP PE

产品名称

Anti-PKC beta 2抗体[Y125]
参阅全部 PKC beta 2 一抗
描述

兔单克隆抗体[Y125] to PKC beta 2
宿主

Rabbit
特异性

This antibody recognises PKC beta 2, it does not cross react with human PKC beta 1. Immunogen's homology with PKC alpha is 77.8%. Cross-reactivity with PKC alpha has not been tested.
经测试应用

适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
种属反应性

与反应: Mouse, Rat, Human, Recombinant fragment
预测可用于: Cow, Pig
免疫原

Synthetic peptide within Human PKC beta 2 aa 600-700 (C terminal). The exact sequence is proprietary.
表位

ab32026 reacts with an epitope located in the region near the C terminus of PKC beta 2.
阳性对照
- WB: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate unboiled, Mouse brain lysate unboiled, Rat brain lysate unboiled, Active GST-tagged human PKC beta 2 full length protein. IHC-P: human gastric carcinoma, mouse cerebrum, rat cerebrum. ICC/IF: HeLa (Human cervix adenocarcinoma epithelial cell) Flow cyto(intra): K-562 (Human chronic myelogenous leukemia lymphoblast) cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

Y125
同种型

IgG
研究领域
- Neuroscience
- Processes

Alternative Versions
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
Recombinant Protein
- Recombinant human PKC beta 2 protein (ab60841)

The Abpromise guarantee

Abpromise™承诺保证使用ab32026于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(4)	1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P	(1)	Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.
Flow Cyt (Intra)		1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

说明
WB 1/1000 - 1/10000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
Flow Cyt (Intra) 1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

功能

Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
序列相似性

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.
翻译后修饰

Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
细胞定位

Cytoplasm. Nucleus. Membrane.
Target information above from: UniProt accession P05771 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 5579 Human
- Entrez Gene: 18751 Mouse
- Entrez Gene: 25023 Rat
- Omim: 176970 Human
- SwissProt: P05126 Cow
- SwissProt: P05771 Human
- SwissProt: P68404 Mouse
- SwissProt: P68403 Rat
- Unigene: 460355 Human
- Unigene: 207496 Mouse
- Unigene: 446371 Mouse
- Unigene: 91118 Rat
see all
别名
- KPCB_HUMAN antibody
- PKC Beta antibody
- PKC-B antibody
- PKC-beta antibody
- PKCB antibody
- Prkcb antibody
- PRKCB II antibody
- PRKCB2 antibody
- Protein kinase C beta antibody
- Protein kinase C beta type antibody
see all

Flow Cytometry (Intracellular) - Anti-PKC beta 2 antibody [Y125] (ab32026)

Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling PKC beta 2 with purified ab32026 at 1/20 dilution (5 ug/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
Immunocytochemistry/ Immunofluorescence - Anti-PKC beta 2 antibody [Y125] (ab32026)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PKC beta 2 using ab32026. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab32026 at 1:50 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)

Immunohistochemical analysis of Paraffin-embedded sections rat cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on rat cerebrum tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)

Immunohistochemical analysis of Paraffin-embedded sections mouse cerebrum tissue labelling PKC beta 2 with ab32026 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on mouse cerebrum tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC beta 2 antibody [Y125] (ab32026)

Immunohistochemical analysis of Paraffin-embedded sections human gastric carcinoma tissue labelling PKC beta 2 with ab32026 at 1/300 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human gastric carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)

All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution

Lane 1 : Recombinant human PKC beta 2 protein (ab60841)
Lane 2 : Recombinant human PKC beta 1 protein (ab60840)
Lane 3 : Recombinant human PKC delta protein (ab60844)
Lane 4 : Recombinant human PKC eta protein (ab60849)
Lane 5 : Recombinant human PKC epsilon protein (ab60847)
Lane 6 : Recombinant human PKC theta/PRKCQ protein (ab56641)
Lane 7 : Recombinant human PKC mu/PKD protein (ab60873)
Lane 8 : Recombinant human PKC alpha protein (Active) (ab55672)
Lane 9 : Recombinant human PKC gamma protein (ab60842)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 77 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

ab235384 was used to detect GST tag.
Western blot - Anti-PKC beta 2 antibody [Y125] (ab32026)

All lanes : Anti-PKC beta 2 antibody [Y125] (ab32026) at 1/1000 dilution

Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate boiled
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate unboiled
Lane 3 : Mouse brain lysate boiled
Lane 4 : Mouse brain lysate unboiled
Lane 5 : Rat brain lysate boiled
Lane 6 : Rat brain lysate unboiled

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

ab181602 was used as GAPDH loading control.

We recommend not to boil samples when loading lysate.
Anti-PKC beta 2 antibody [Y125] (ab32026)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

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发表研究结果有使用 ab32026？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab32026 被引用在 26 文献中.

Nieto-Felipe J et al. The store-operated Ca2+ channel Orai1α is required for agonist-evoked NF-κB activation by a mechanism dependent on PKCβ2. J Biol Chem 299:102882 (2023). PubMed: 36623731
Zhao L et al. Dendritic cell-mediated chronic low-grade inflammation is regulated by the RAGE-TLR4-PKCβ1 signaling pathway in diabetic atherosclerosis. Mol Med 28:4 (2022). PubMed: 35062863
Kim JM et al. Downregulation of matriptase suppresses the PAR‑2/PLCγ2/PKC‑mediated invasion and migration abilities of MCF‑7 breast cancer cells. Oncol Rep 46:N/A (2021). PubMed: 34608498
Child SJ et al. Rapid adaptation to human protein kinase R by a unique genomic rearrangement in rhesus cytomegalovirus. PLoS Pathog 17:e1009088 (2021). PubMed: 33497413
Kim JM et al. Bruton's agammaglobulinemia tyrosine kinase (Btk) regulates TPA-induced breast cancer cell invasion via PLC?2/PKCß/NF-?B/AP-1-dependent matrix metalloproteinase-9 activation. Oncol Rep 45:N/A (2021). PubMed: 33760219

View all Publications for this product

客户评价及客户问答

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1-7 of 7 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (lymph node abscess)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Leica ER-1 low pH buffer

Permeabilization

Yes - Tween-20

Specification

lymph node abscess

Blocking step

(agent) for 40 minute(s) · Concentration: 100%

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Jan 05 2022

Application

Western blot

Sample

Human Tissue lysate - whole (Brain lysate)

Gel Running Conditions

Reduced Denaturing (12%)

Loading amount

15 µg

Specification

Brain lysate

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 20 2018

Application

Immunohistochemistry (Frozen sections)

Sample

Pig Tissue sections (brain)

Permeabilization

Specification

brain

Blocking step

H202 as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 23°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 09 2017

Application

Western blot

Sample

Cow Cell lysate - other (Cerebral microvascular endothelial cells)

Gel Running Conditions

Reduced Denaturing (8% Bis-Tris)

Loading amount

10 µg

Specification

Cerebral microvascular endothelial cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7.5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 14 2015

Application

Western blot

Sample

Human Cell lysate - whole cell (MCF7)

Loading amount

100000 cells

Specification

MCF7

Gel Running Conditions

Reduced Denaturing

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 May 12 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (GLC16 Cells)

Loading amount

40000 cells

Specification

GLC16 Cells

Gel Running Conditions

Reduced Denaturing (10% SDS-PAGE)

Blocking step

Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 10 2010

Question

Thanks a lot for your kindly reply. I want to use this antibody for Co-IP, so I will report to my PI and feedback later.

Best Regards

Abcam community

Verified customer

Asked on May 30 2012

Answer

Thank you for contacting us.

This antibody has been tested in IP so it will more likely to work in Co-IP also. However we cannot confirm it because we do not have tested data. The experimental conditions have to be determined by the end user.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

回复于 May 30 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

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概述

产品名称

描述

宿主

特异性

经测试应用

种属反应性

免疫原

表位

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (26)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC beta 2 antibody [Y125]

Western blot abreview for Anti-PKC beta 2 antibody [Y125]

Immunohistochemistry (Frozen sections) abreview for Anti-PKC beta 2 antibody [Y125]

Western blot abreview for Anti-PKC beta 2 antibody [Y125]

Western blot abreview for Anti-PKC beta 2 antibody [Y125]

Western blot abreview for Anti-PKC beta 2 antibody [Y125]