Anti-PKC alpha (phospho S657 + Y658)抗体(ab23513)
Key features and details
- Rabbit polyclonal to PKC alpha (phospho S657 + Y658)
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG
概述
-
产品名称
Anti-PKC alpha (phospho S657 + Y658)抗体
参阅全部 PKC alpha 一抗 -
描述
兔多克隆抗体to PKC alpha (phospho S657 + Y658) -
宿主
Rabbit -
特异性
ab23513 detected PKC alpha and PKC beta I, but not other PKC isoforms in rat brain lysate. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Human PKC alpha (phospho S657 + Y658).
-
常规说明
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
-
纯度
Affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC |
Use at an assay dependent concentration.
|
|
WB | (4) |
1/1000. Detects a band of approximately 82 kDa.
|
说明 |
---|
ICC
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 82 kDa. |
靶标
-
功能
This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
序列相似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
细胞定位
Cytoplasm. Cell membrane. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 24680 Rat
- SwissProt: P05696 Rat
- Unigene: 207908 Rat
-
别名
- AAG6 antibody
- Aging associated gene 6 antibody
- aPKC antibody
see all
图片
-
Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658).
-
Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKCα (Ser-657/Tyr-658) (PP1091) antibody in the absence (Left) or presence (Right) of blocking peptide (PX1095). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594
-
Immunocytochemistry - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)Image from Taniuchi K et al., PLoS One. 2012;7(4):e35674. Epub 2012 Apr 19. Fig 8.; doi:10.1371/journal.pone.0035674; April 19, 2012, PLoS ONE 7(4): e35674.Immunofluorescence analysis of S2-013 cells, staining PKC alpha (phospho S657 + Y658) with ab23513.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with blocking solution (3% BSA/PBS). Cells were incubated with primary antibody for 1 hour. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (22)
ab23513 被引用在 22 文献中.
- Teegala LR et al. Protein Kinase C α and β compensate for each other to promote stem cell factor-mediated KIT phosphorylation, mast cell viability and proliferation. FASEB J 36:e22273 (2022). PubMed: 35349200
- Jiang Y et al. Aquaporin 1 mediates early responses to osmotic stimuli in endothelial cells via the calmodulin pathway. FEBS Open Bio 11:75-84 (2021). PubMed: 33125833
- Wu A et al. Delphinidin induces cell cycle arrest and apoptosis in HER-2 positive breast cancer cell lines by regulating the NF-κB and MAPK signaling pathways. Oncol Lett 22:832 (2021). PubMed: 34712357
- Komleva YK et al. NLRP3 Inflammasome Blocking as a Potential Treatment of Central Insulin Resistance in Early-Stage Alzheimer's Disease. Int J Mol Sci 22:N/A (2021). PubMed: 34769018
- Lenkavska L et al. Searching for combination therapy by clustering methods: Stimulation of PKC in Golgi apparatus combined with hypericin induced PDT. Photodiagnosis Photodyn Ther N/A:101813 (2020). PubMed: 32442674