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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC

Anti-PKC alpha (phospho S657 + Y658)抗体(ab23513)

  • Datasheet
  • SDS
Reviews (6)Q&A (7)References (22)

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Western blot - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)
  • Immunocytochemistry - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)
  • Immunocytochemistry - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)

Key features and details

  • Rabbit polyclonal to PKC alpha (phospho S657 + Y658)
  • Suitable for: ICC, WB
  • Reacts with: Rat
  • Isotype: IgG

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概述

  • 产品名称

    Anti-PKC alpha (phospho S657 + Y658)抗体
    参阅全部 PKC alpha 一抗
  • 描述

    兔多克隆抗体to PKC alpha (phospho S657 + Y658)
  • 宿主

    Rabbit
  • 特异性

    ab23513 detected PKC alpha and PKC beta I, but not other PKC isoforms in rat brain lysate.
  • 经测试应用

    适用于: ICC, WBmore details
  • 种属反应性

    与反应: Rat
  • 免疫原

    Synthetic peptide corresponding to Human PKC alpha (phospho S657 + Y658).

  • 常规说明

    We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human PKC alpha protein (Active) (ab55672)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab23513于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
WB (4)
1/1000. Detects a band of approximately 82 kDa.
说明
ICC
Use at an assay dependent concentration.
WB
1/1000. Detects a band of approximately 82 kDa.

靶标

  • 功能

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 细胞定位

    Cytoplasm. Cell membrane. Nucleus.
  • Target information above from: UniProt accession P17252 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 24680 Rat
    • SwissProt: P05696 Rat
    • Unigene: 207908 Rat
    • 别名

      • AAG6 antibody
      • Aging associated gene 6 antibody
      • aPKC antibody
      • KPCA_HUMAN antibody
      • PKC alpha antibody
      • PKC-A antibody
      • PKC-alpha antibody
      • PKCA antibody
      • PRKACA antibody
      • PRKCA antibody
      • Protein Kinase C alpha antibody
      • Protein kinase C alpha type antibody
      see all

    图片

    • Western blot - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)
      Western blot - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)

      Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658).

    • Immunocytochemistry - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)
      Immunocytochemistry - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)

      Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKCα (Ser-657/Tyr-658) (PP1091) antibody in the absence (Left) or presence (Right) of blocking peptide (PX1095). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594

    • Immunocytochemistry - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)
      Immunocytochemistry - Anti-PKC alpha (phospho S657 + Y658) antibody (ab23513)Image from Taniuchi K et al., PLoS One. 2012;7(4):e35674. Epub 2012 Apr 19. Fig 8.; doi:10.1371/journal.pone.0035674; April 19, 2012, PLoS ONE 7(4): e35674.
      Immunofluorescence analysis of S2-013 cells, staining PKC alpha (phospho S657 + Y658) with ab23513.

      Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with blocking solution (3% BSA/PBS). Cells were incubated with primary antibody for 1 hour. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (22)

    发表研究结果有使用 ab23513?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab23513 被引用在 22 文献中.

    • Teegala LR  et al. Protein Kinase C α and β compensate for each other to promote stem cell factor-mediated KIT phosphorylation, mast cell viability and proliferation. FASEB J 36:e22273 (2022). PubMed: 35349200
    • Jiang Y  et al. Aquaporin 1 mediates early responses to osmotic stimuli in endothelial cells via the calmodulin pathway. FEBS Open Bio 11:75-84 (2021). PubMed: 33125833
    • Wu A  et al. Delphinidin induces cell cycle arrest and apoptosis in HER-2 positive breast cancer cell lines by regulating the NF-κB and MAPK signaling pathways. Oncol Lett 22:832 (2021). PubMed: 34712357
    • Komleva YK  et al. NLRP3 Inflammasome Blocking as a Potential Treatment of Central Insulin Resistance in Early-Stage Alzheimer's Disease. Int J Mol Sci 22:N/A (2021). PubMed: 34769018
    • Lenkavska L  et al. Searching for combination therapy by clustering methods: Stimulation of PKC in Golgi apparatus combined with hypericin induced PDT. Photodiagnosis Photodyn Ther N/A:101813 (2020). PubMed: 32442674
    View all Publications for this product

    客户评价及客户问答

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    1-10 of 13 Abreviews or Q&A

    Western blot abreview for Anti-PKC alpha (phospho S657 + Y658) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Gastric Cancer Cell Line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    3 µg
    Specification
    Gastric Cancer Cell Line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 04 2020

    Western blot abreview for Anti-PKC alpha (phospho S657 + Y658) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Macrophage)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    3 µg
    Specification
    Macrophage
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Nov 03 2020

    Western blot abreview for Anti-PKC alpha (phospho S657 + Y658) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (MDA-MB-231)
    Loading amount
    35 µg
    Specification
    MDA-MB-231
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 09 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PKC alpha (phospho S657 + Y658) antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse preneoplastic leasions (PanIN))
    Specification
    mouse preneoplastic leasions (PanIN)
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate pH=6
    Permeabilization
    Yes - PBST
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Feb 04 2011

    Question

    I received the antibody ab72155 this morning. This is really efficient. Thanks a lot.

    This antibody requires the antigen retrieval procedure. Do you have protocol for the antigen retrieval?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Thank you for your reply, and I am glad that you got the replacement antibody. We always ship our products overnight, so you can continue your research as quickly as possible.

    For ab72155, we recommend starting with heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins.

    If there is anything else I can help you with, or if you have any issues with the new antibody, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Aug 08 2012

    Question

    We still could not get this PKC antibody working on WB. May I ask a replacement for this antibody. I would like to get anti-TRPM7 antibody, the catalog number is ab72155.


    Thanks a lot for taking care of this issue.

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Thank you for your reply.

    I am sorry about the problems you have been having with ab23513 in western blot. As requested, I am sending you a vial of ab72155 as a free of charge replacement. For your records, your new order number is *.

    If there is anything else I can help you with, or if you have any issues with the new antibody, please let me know.

    Read More

    Abcam Scientific Support

    回复于 Aug 08 2012

    Question

    We are trying to detect the phosphorylation level of PKC in the treated primary human dental epithelial cells by Western Blot. Do you know any other anti-phosphorylated PKC antibody may work for our purpose?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2012

    Answer

    Thank you for your reply.

    I cannot find any other antibodies that detect phosphorylated PKC on residues S657 & Y658, but we have antibodies that detected other phosphorylated residues of PKC. There are links to these below:

    https://www.abcam.com/PKC-alpha-phospho-T497-antibody-EP2608Y-ab76016.html
    https://www.abcam.com/PKC-alpha-phospho-T638-antibody-E195-ab32502.html



    I would be more than happy to send you either of these antibodies as a replacement, just let me know which one you would like to receive. If you could also let me know either the Abcam order number or PO number that was used to purchase this antibody, then that would be very helpful to.

    I look forward to your reply.

    Read More

    Abcam Scientific Support

    回复于 Jul 03 2012

    Question

    Thanks for following up this issue. We ordered the antibody ab23513 last month, and used it to run Western Blot on human cells. We modified protocol after speaking to you last time. However, the problems are still remained. We saw more than one band in the result, and the size of these bands are less than 62 KD. I attached one of these films for your consideration. Please let me know if you have more questions.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 26 2012

    Answer

    Thank you for your reply.

    I am sorry that the antibody is still not working for you and so I would be happy to either replace or refund it for you.

    If you would like a replacement antibody, then please let me know which antibody you would like to receive and if you could provide me with the Abcam order number or the PO# that was used to purchase the antibody, that would be helpful. Or if you would prefer a refund, just let me know.

    I look forward to your reply.

    Read More

    Abcam Scientific Support

    回复于 Jun 26 2012

    Question

    Seeing multiply bands in WB using human cells.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 15 2012

    Answer

    Thank you for calling yesterday.

    I am looking into the issues that you are having with ab23513 in western blot and I was wandering if you would be able to send an image of the results that you are getting. This will help me compare what you are seeing with the lab testing.

    I would just like to assure you that the antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    I look forward to your reply.

    Read More

    Abcam Scientific Support

    回复于 Jun 15 2012

    Question

    merci de bien vouloir trouver ci-après la liste des anticorps pour commande, je vous serais reconnaissante de bien vouloir m’indiquer pour chaque ref citée le code de remise correspondant afin que je puisse passer la commande ferme.

    Par ailleurs, les anticorps référencés ci-dessous, qui ont été acheté à un prix normal, n’ont pas répondu avec le robot SIMON, pourriez-vous envisager un geste commercial pour ces produits qui finalement ont été achetés en vain!

    Read More

    Abcam community

    Verified customer

    Asked on Mar 06 2012

    Answer

    Codes offre de test :
    ab32515 : *****
    ab32096 : *****
    ab7217 : *****
    ab12135 : *****
    ab1791 : *****

    Date d’expiration : JJ MM AAAA

    Je suis ravi que vous souhaitiez participer ànotre offre et tester les anticorps listés ci-dessusavec le robot Simon de Protein Simple.Ces codes de réductionsont utilisables sous 120 jours et pour l’achat d’autres anticorps primaires.

    Vous devez dans un premier temps soumettre une Abreview avec vos résultats enspécifiant le code promotionnel correspondant à l'anticorps testé. Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de l'anticorps testé.

    Concernantles produits pour lesquels vousavez déjà obtenu des résultats, merci dem'envoyer vos données, en échange nous vous attribueronsdes"Abpoints" utilisables pour des remisessur vos commandes ouen bons d'achatAmazon.

    Je reste à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview je vous souhaite unebonne continuation dans vos recherches.

    Read More

    Abcam Scientific Support

    回复于 Mar 06 2012

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