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使用敲除细胞株进行验证重组

重组Anti-PKC alpha抗体[133] (ab11723)

Reviews (1) Submit a question References (20)
Western blot - Anti-PKC alpha antibody [133] (ab11723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)
  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)
  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [133] to PKC alpha
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-PKC alpha抗体[133]
    参阅全部 PKC alpha 一抗

  • 描述

    小鼠单克隆抗体[133] to PKC alpha

  • 宿主

    Mouse

  • 特异性

    ab11723 recognises the alpha isoform of PKC.

  • 经测试应用

    适用于: WB, IHC-Pmore details
    不适用于: ICC or ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab55672)

  • 阳性对照

    • WB: HAP1, K562, C6, HeLa, NIH/3T3 and HEK-293 cell lysate. Human kidney tissue lysate. Mouse and rat brain and spleen tissue lysate. Recombinant human PKC alpha protein (Active) (ab55672). IHC-P: Human, mouse and rat retina tissue.

  • 常规说明

    This product has switched from a hybridoma to recombinant production method on 25th July 2021.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab11723于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/1000. Predicted molecular weight: 76 kDa.Can be blocked with Recombinant human PKC alpha protein (Active) (ab55672).
IHC-P
1/1 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
WB
1/500 - 1/1000. Predicted molecular weight: 76 kDa.Can be blocked with Recombinant human PKC alpha protein (Active) (ab55672).
IHC-P
1/1 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for ICC or ICC/IF.

靶标

  • 功能

    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

  • 序列相似性

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.

  • 细胞定位

    Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • AAG6 antibody
    • Aging associated gene 6 antibody
    • aPKC antibody
    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PRKACA antibody
    • PRKCA antibody
    • Protein Kinase C alpha antibody
    • Protein kinase C alpha type antibody
    see all

图片

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)

    This image was generated from the hybridoma version of the product.

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: K562 cell lysate (20 µg)
    Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)

    Immunohistochemical analysis of Paraffin-embedded human retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on human retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

     

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)

    This image was generated from the hybridoma version of the product.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)
    All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

    Lane 1 : Recombinant human PKC alpha protein (Active) (ab55672)
    Lane 2 : Recombinant human PKC beta 1 protein (ab60840)
    Lane 3 : Recombinant human PKC beta 2 protein (ab60841)
    Lane 4 : Recombinant human PKC eta protein (ab60849)
    Lane 5 : Recombinant human PKC epsilon protein (ab60847)
    Lane 6 : Recombinant human PKC theta/PRKCQ protein (ab56641)
    Lane 7 : Recombinant human PKC mu/PKD protein (ab60873)
    Lane 8 : Recombinant human PKC zeta protein (ab60848)
    Lane 9 : Recombinant human PKC iota protein (ab60850)
    Lane 10 : Recombinant human PKC gamma protein (ab60842)

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 76 kDa



    5% NFDM/TBST

    All positive controls 10 ng

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)
    All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

    Lane 1 : Recombinant human PKC alpha protein (Active) (ab55672)
    Lane 2 : Recombinant human PKC delta protein (ab60844)

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 76 kDa


    Exposure time: 180 seconds


    5% NFDM/TBST

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on mouse retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)
    All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate 20 ug
    Lane 2 : Mouse brain tissue lysate 20 ug
    Lane 3 : Mouse spleen tissue lysate 20 ug
    Lane 4 : Rat brain tissue lysate 20 ug
    Lane 5 : Rat spleen tissue lysate 20 ug

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 76 kDa


    Exposure time: 180 seconds


    5% NFDM/TBST

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)
    All lanes : Anti-PKC alpha antibody [133] (ab11723) at 1/1000 dilution

    Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate 20 µg
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate 20 µg
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 76 kDa



    Exposure time

    Lane 1: 136 seconds
    Lane 2 and 3: 15 seconds

    Fresh lysates were used in lane 2 and 3.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha antibody [133] (ab11723)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labelling PKC alpha with ab11723 at 1/500 dilution, followed by ready to use secondary antibody Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Positive staining on rat retina is observed. Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Goat Anti-Mouse IgG H&L (HRP polymer) ab214879.

文献 (20)

发表研究结果有使用 ab11723？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab11723 被引用在 20 文献中.

  • Bouslama R  et al. Phosphorylation of PACSIN2 at S313 Regulates Podocyte Architecture in Coordination with N-WASP. Cells 12:N/A (2023). PubMed: 37296607
  • Zhu QJ  et al. PRKCA Promotes Mitophagy through the miR-15a-5p/PDK4 Axis to Relieve Sepsis-Induced Acute Lung Injury. Infect Immun 91:e0046522 (2023). PubMed: 36448837
  • Chen S  et al. Human Umbilical Cord Blood-Derived CD133+CD34+ Cells Protect Retinal Endothelial Cells and Ganglion Cells in X-Irradiated Rats through Angioprotective and Neurotrophic Factors. Front Cell Dev Biol 10:801302 (2022). PubMed: 35223834
  • Hu S  et al. In Vivo CRISPR/Cas9-Mediated Genome Editing Mitigates Photoreceptor Degeneration in a Mouse Model of X-Linked Retinitis Pigmentosa. Invest Ophthalmol Vis Sci 61:31 (2020). PubMed: 32330228
  • Guo Y  et al. Modeling Retinitis Pigmentosa: Retinal Organoids Generated From the iPSCs of a Patient With the USH2A Mutation Show Early Developmental Abnormalities. Front Cell Neurosci 13:361 (2019). PubMed: 31481876
View all Publications for this product

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Western blot abreview for Anti-PKC alpha antibody [133]

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Dec 05 2005

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