Anti-PKA R2/PKR2 (phospho S96)抗体(ab226754)
Key features and details
- Rabbit polyclonal to PKA R2/PKR2 (phospho S96)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PKA R2/PKR2 (phospho S96)抗体
参阅全部 PKA R2/PKR2 一抗 -
描述
兔多克隆抗体to PKA R2/PKR2 (phospho S96) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee
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免疫原
Synthetic peptide within Human PKA R2/PKR2 (N terminal) (phospho S96). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Conjugated to a carrier protein.
Database link: P13861 -
阳性对照
- WB: HEK-293T whole cell extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226754于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
|
| 说明 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
靶标
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功能
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. -
组织特异性
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. -
序列相似性
Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains. -
翻译后修饰
Phosphorylated by the activated catalytic chain. -
细胞定位
Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5576 Human
- Omim: 176910 Human
- SwissProt: P13861 Human
- Unigene: 631923 Human
- Unigene: 731573 Human
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别名
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
see all
图片
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Western blot - Anti-PKA R2/PKR2 (phospho S96) antibody (ab226754)All lanes : Anti-PKA R2/PKR2 (phospho S96) antibody (ab226754) at 1/3000 dilution
Lane 1 : Untreated HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : CIP treated HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 46 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab226754 被引用在 1 文献中.
- Peng L et al. Prostaglandin E2 Receptor 4 (EP4) Affects Trophoblast Functions via Activating the cAMP-PKA-pCREB Signaling Pathway at the Maternal-Fetal Interface in Unexplained Recurrent Miscarriage. Int J Mol Sci 22:N/A (2021). PubMed: 34502044