Key features and details
- Rabbit polyclonal to PKA R2/PKR2
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
参阅全部 PKA R2/PKR2 一抗
描述兔多克隆抗体to PKA R2/PKR2
特异性ab38949 binds to PKARIIa, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIb, PKARIIb) and can recognize native or reduced PKARIIa.
经测试应用适用于: ICC/IFmore details
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
纯度Immunogen affinity purified
纯化说明ab38949 has been peptide-affinity purified.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
The Abpromise guarantee
Use at an assay dependent concentration.
Use at an assay dependent concentration.
功能Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
组织特异性Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
序列相似性Belongs to the cAMP-dependent kinase regulatory chain family.
Contains 2 cyclic nucleotide-binding domains.
翻译后修饰Phosphorylated by the activated catalytic chain.
细胞定位Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
- Information by UniProt
- cAMP dependent protein kinase regulatory subunit alpha 2 antibody
- cAMP dependent protein kinase regulatory subunit RII alpha antibody
- cAMP dependent protein kinase type II alpha regulatory chain antibody
ICC/IF image of ab38949 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ICC/IF image of ab38949 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab38949 被引用在 17 文献中.
- LaCroix R et al. Complex effects of kinase localization revealed by compartment-specific regulation of protein kinase A activity. Elife 11:N/A (2022). PubMed: 35199643
- Zhan Y et al. Maren Pills Improve Constipation via Regulating AQP3 and NF-?B Signaling Pathway in Slow Transit Constipation In Vitro and In Vivo. Evid Based Complement Alternat Med 2020:9837384 (2020). PubMed: 32774435
- Xiao J et al. Protective Effects of Adiponectin against Cobalt Chloride-Induced Apoptosis of Smooth Muscle Cells via cAMP/PKA Pathway. Biomed Res Int 2020:7169348 (2020). PubMed: 33102590
- Zhang F et al. Effect of different charges of modified electroconvulsive seizure on the cognitive behavior in stressed rats: Effects of GluR1 phosphorylation and CaMKIIa activity. Exp Ther Med 17:748-758 (2019). PubMed: 30651859
- Chen Q et al. Subcellular localization of aquaporin 3 in prostate cancer is regulated by RalA. Oncol Rep 39:2171-2177 (2018). PubMed: 29532894