Anti-PIWIL4/PIWI抗体(ab111714)
Key features and details
- Rabbit polyclonal to PIWIL4/PIWI
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIWIL4/PIWI抗体
参阅全部 PIWIL4/PIWI 一抗 -
描述
兔多克隆抗体to PIWIL4/PIWI -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Monkey
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免疫原
Recombinant fragment corresponding to Human PIWIL4/PIWI aa 639-839. (AAH31060)
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阳性对照
- MCF7 and IMR32 cells and whole cell lysates; 59T xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab111714 is purified by antigen-affinity chromatography. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab111714于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 97 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 97 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0) |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. -
组织特异性
Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed. -
序列相似性
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
翻译后修饰
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. -
细胞定位
Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization. - Information by UniProt
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数据库链接
- Entrez Gene: 143689 Human
- Omim: 610315 Human
- SwissProt: Q7Z3Z4 Human
- Unigene: 660188 Human
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别名
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
see all
图片
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Western blot - Anti-PIWIL4/PIWI antibody (ab111714)Anti-PIWIL4/PIWI antibody (ab111714) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 97 kDa
7.5% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL4/PIWI antibody (ab111714)ab111714 at 1/200 dilution staining PIWIL4/PIWI in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL4/PIWI antibody (ab111714)ab111714 at 1/500 dilution staining PIWIL4/PIWI in Formalin-fixed, paraffin-embedded 59T xenograft tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab111714 被引用在 10 文献中.
- Abdelhamid RF et al. piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis. Mol Neurobiol 59:1693-1705 (2022). PubMed: 35015250
- Xie Q et al. piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner. J Transl Med 20:51 (2022). PubMed: 35093098
- Liu J et al. piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification. Aging (Albany NY) 13:23361-23375 (2021). PubMed: 34645714
- Singh G et al. Genome-wide profiling of the PIWI-interacting RNA-mRNA regulatory networks in epithelial ovarian cancers. PLoS One 13:e0190485 (2018). PubMed: 29320577
- Gebert M et al. PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells. Sci Rep 8:16431 (2018). PubMed: 30401887
- Jacobs DI et al. piRNA-8041 is downregulated in human glioblastoma and suppresses tumor growth in vitro and in vivo. Oncotarget 9:37616-37626 (2018). PubMed: 30701019
- Roy J & Mallick B Investigating piwi-interacting RNA regulome in human neuroblastoma. Genes Chromosomes Cancer 57:339-349 (2018). PubMed: 29516567
- Wang Z et al. The Role of PIWIL4, an Argonaute Family Protein, in Breast Cancer. J Biol Chem 291:10646-58 (2016). PubMed: 26957540
- Cordeiro A et al. PiwiRNA-651 as marker of treatment response and survival in classical Hodgkin lymphoma. Oncotarget 7:46002-46013 (2016). PubMed: 27329591
- Hashim A et al. RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Oncotarget 5:9901-10 (2014). PubMed: 25313140