Anti-PIWIL3抗体(ab77088)
Key features and details
- Mouse polyclonal to PIWIL3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIWIL3抗体
参阅全部 PIWIL3 一抗 -
描述
小鼠多克隆抗体to PIWIL3 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length human PIWIL3 (NP_001008496, aa 1-882).
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阳性对照
- PIWIL3 transfected 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77088于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
靶标
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功能
May play a role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. -
组织特异性
Expressed in testis. -
序列相似性
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
细胞定位
Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
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数据库链接
- Entrez Gene: 440822 Human
- Omim: 610314 Human
- SwissProt: Q7Z3Z3 Human
- Unigene: 448343 Human
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别名
- HIWI3 antibody
- Piwi like 3 antibody
- Piwi-like protein 3 antibody
see all
图片
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Western blot - Anti-PIWIL3 antibody (ab77088)All lanes : Anti-PIWIL3 antibody (ab77088) at 5 µg/ml
Lane 1 : PIWIL3 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 97 kDa
Observed band size: 97 kDa
Additional bands at: 40 kDa, 60 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
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Datasheet download
文献 (3)
ab77088 被引用在 3 文献中.
- Abdelhamid RF et al. piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis. Mol Neurobiol 59:1693-1705 (2022). PubMed: 35015250
- Liu J et al. piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification. Aging (Albany NY) 13:23361-23375 (2021). PubMed: 34645714
- Hashim A et al. RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer. Oncotarget 5:9901-10 (2014). PubMed: 25313140
