Anti-PIWIL2抗体(ab181340)
Key features and details
- Rabbit polyclonal to PIWIL2
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIWIL2抗体
参阅全部 PIWIL2 一抗 -
描述
兔多克隆抗体to PIWIL2 -
宿主
Rabbit -
特异性
At least three isoforms of PIWIL2 are known to exist; ab181340 will only detect the two longest isoforms. ab181340 is predicted to not cross-react with other PIWI protein family members. -
经测试应用
适用于: WB, ICC, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide within Human PIWIL2 (internal sequence). The exact sequence is proprietary. Immunogen is 17 amino acids synthetic peptide within amino acids 410-460.
Database link: Q8TC59 -
阳性对照
- HepG2 cell lysate; HepG2 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181340于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa.
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ICC |
Use a concentration of 2.5 µg/ml.
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ICC/IF | (1) |
Use a concentration of 5 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 110 kDa. |
ICC
Use a concentration of 2.5 µg/ml. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate to similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. -
组织特异性
Expressed in adult testis and in most tumors. -
序列相似性
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
翻译后修饰
Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein TDRD1 and subsequent localization to the meiotic nuage, also named P granule. -
细胞定位
Cytoplasm. Present in chromatoid body. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. - Information by UniProt
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数据库链接
- Entrez Gene: 55124 Human
- Entrez Gene: 57746 Mouse
- Omim: 610312 Human
- SwissProt: Q8TC59 Human
- SwissProt: Q8CDG1 Mouse
- Unigene: 614809 Human
- Unigene: 85253 Mouse
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别名
- Cancer/testis antigen 80 antibody
- CT80 antibody
- Hiwi like antibody
see all
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab181340 被引用在 10 文献中.
- Abdelhamid RF et al. piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis. Mol Neurobiol 59:1693-1705 (2022). PubMed: 35015250
- Liu J et al. piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification. Aging (Albany NY) 13:23361-23375 (2021). PubMed: 34645714
- Tharp ME et al. Maximizing the ovarian reserve in mice by evading LINE-1 genotoxicity. Nat Commun 11:330 (2020). PubMed: 31949138
- Zhang D et al. Exosomes derived from Piwil2-induced cancer stem cells transform fibroblasts into cancer-associated fibroblasts. Oncol Rep 43:1125-1132 (2020). PubMed: 32323829
- Greeson KW et al. Detrimental effects of flame retardant, PBB153, exposure on sperm and future generations. Sci Rep 10:8567 (2020). PubMed: 32444626
- Subhramanyam CS et al. Role of PIWI-like 4 in modulating neuronal differentiation from human embryonal carcinoma cells. RNA Biol 17:1613-1624 (2020). PubMed: 32372724
- Li W et al. The Prognosis Value of PIWIL1 and PIWIL2 Expression in Pancreatic Cancer. J Clin Med 8:N/A (2019). PubMed: 31443431
- Gebert M et al. PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells. Sci Rep 8:16431 (2018). PubMed: 30401887
- Giebler M et al. Differential Regulation of PIWI-LIKE 2 Expression in Primordial Germ Cell Tumor Cell Lines by Promoter Methylation. Front Genet 9:375 (2018). WB ; Human . PubMed: 30294341
- Cao J et al. High expression of piwi-like RNA-mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-ß receptors and cyclin-dependent kinases in breast cancer. Mol Med Rep 13:2829-35 (2016). PubMed: 26847393