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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing RNAi

Anti-Piwi抗体(ab5207)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (8)

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Western blot - Anti-Piwi antibody (ab5207)
  • Western blot - Anti-Piwi antibody (ab5207)

Key features and details

  • Rabbit polyclonal to Piwi
  • Suitable for: WB
  • Reacts with: Drosophila melanogaster
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Piwi抗体
  • 描述

    兔多克隆抗体to Piwi
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WBmore details
  • 种属反应性

    与反应: Drosophila melanogaster
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • This antibody gave a positive signal in Drosophila embryo nuclear extract
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • RNAi
    • Epigenetics and Nuclear Signaling
    • RNAi
    • RISC
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Argonaute and Piwi
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Nuclear Pore Complex

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5207于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
说明
应用说明
WB: 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 97 kDa). Can be blocked with Piwi peptide (ab14803).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 相关性

    Piwi mediates a somatic signaling mechanism required for the maintenance of germline stem cells to produce and maintain a daughter germline stem cell. It is not essential for the further differentiation of the committed daughter cell. Acts cell autonomously to promote germline stem cell division. It is expressed somatically in the ovariole terminal filament and epithelial sheath cells. Expressed in the germarium at low levels during oogenesis stages 1-6, at a lower level during stages 7-9, strongly at stage 10 and eventually accumulating in early embryos.
  • 细胞定位

    Nuclear
  • 数据库链接

    • Entrez Gene: 34521 Drosophila melanogaster
    • SwissProt: Q9VKM1 Drosophila melanogaster
    • 别名

      • P-element induced wimpy testis antibody
      • Piwi antibody

    图片

    • Western blot - Anti-Piwi antibody (ab5207)
      Western blot - Anti-Piwi antibody (ab5207)
      Anti-Piwi antibody (ab5207) at 1 µg/ml + Drosophila embryo nuclear extract (from melanogaster embryos 0-12Hr) at 20 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size: 97 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?
      Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-Piwi antibody (ab5207)
      Western blot - Anti-Piwi antibody (ab5207)This image is courtesy of Ahmet Denli

      Drosophila embryo lysate, 8% TRIS SDS-PAGE.
      The arrow indicates Piwi at a predicted MW of 90 kDa.
      Pre-immune sera was used as a control and showed no equivalent band (data not shown).

      Drosophila embryo lysate, 8% TRIS SDS-PAGE. The arrow indicates Piwi at a predicted MW of 90 kDa. Pre-immune sera was used as a control and showed no equivalent band (data not shown). Ahmet Denli

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (8)

    发表研究结果有使用 ab5207?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5207 被引用在 8 文献中.

    • Jiménez-Merino J  et al. Putative stem cells in the hemolymph and in the intestinal submucosa of the solitary ascidian Styela plicata. Evodevo 10:31 (2019). PubMed: 31788180
    • Jeffery WR Distal Regeneration Involves the Age Dependent Activity of Branchial Sac Stem Cells in the Ascidian Ciona intestinalis. Regeneration (Oxf) 2:1-18 (2015). PubMed: 25893097
    • Patil VS  et al. The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster. BMC Biol 12:61 (2014). WB ; Drosophila melanogaster . PubMed: 25287931
    • Dufourt J  et al. Spatio-temporal requirements for transposable element piRNA-mediated silencing during Drosophila oogenesis. Nucleic Acids Res N/A:N/A (2013). IHC-P ; Drosophila melanogaster . PubMed: 24288375
    • Moshkovich N  et al. RNAi-independent role for Argonaute2 in CTCF/CP190 chromatin insulator function. Genes Dev 25:1686-701 (2011). WB ; Drosophila melanogaster . PubMed: 21852534
    • Klenov MS  et al. Separation of stem cell maintenance and transposon silencing functions of Piwi protein. Proc Natl Acad Sci U S A 108:18760-5 (2011). PubMed: 22065765
    • Brown FD  et al. Whole body regeneration in a colonial ascidian, Botrylloides violaceus. J Exp Zool B Mol Dev Evol 312:885-900 (2009). PubMed: 19588490
    • Rosner A  et al. Vasa and the germ line lineage in a colonial urochordate. Dev Biol 331:113-28 (2009). WB . PubMed: 19406116

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Piwi antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Fruit fly (Drosophila melanogaster) Cell (ovaries)
    Specification
    ovaries
    Permeabilization
    Yes - Triton 0,3%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Ms. Nathalie Gred Gueguen

    Verified customer

    提交于 Oct 08 2014

    Question

    I am writing to hear whether it is possible to buy small aliquots of the following antibodies or whether it would be possible to ask for free aliquots to see if the antibodies work in our model organism, the planarian: *Piwi antibody (ab5207) *Argonaute 4 antibody (ab3600) *Nanos homologue 2 (NANOS2) antibody (ab15731) *Nanos Homologue 1 (NANOS1) antibody (ab15733) I am a PhD student working on a characterization of the planarian stem cells (the neoblasts) and of germline stem cells. The planarian that we are using in the lab is the flatworm Schmidtea mediterranea (phylum Platyhelminthes, class Turbellaria, order Seriata, suborder Tricladida). I have studied the expression pattern of nanos- and piwi-like genes by whole mount in situ hybridization, but would like to try immunostaining with the corresponding antibodies to get a clearer pattern. Since we do not know whether the antibodies that you offer will work on the planarian, we would like to buy/to ask for a small aliquot to try it out before buying the entire sample. If the antibody works, we will buy the entire sample. Other antibodies that we are interested in trying for the characterization of the planarian stem cells and germline stem cells are the following: * Sox2 antibody (ab15830) * Oct4 antibody (ab18976) * Fragilis antibody (ab15592) * Nanog (ab21603) * SCF (ab17521) * VASA (ab13840) * Beclin 1 antibody (ab16998) I am looking forward to hearing from you!

    Read More

    Abcam community

    Verified customer

    Asked on Sep 16 2005

    Answer

    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase one of these products, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image).

    Read More

    Abcam Scientific Support

    回复于 Sep 16 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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