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重组RabMAb

重组Anti-Pit1抗体[EPR23555-203] - BSA and Azide free (ab273057)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Western blot - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Flow Cytometry (Intracellular) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
  • Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23555-203] to Pit1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Pit1抗体[EPR23555-203] - BSA and Azide free
    参阅全部 Pit1 一抗

  • 描述

    兔单克隆抗体[EPR23555-203] to Pit1 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IF, IP, IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: GH3 whole cell lysate, Mouse pituitary tissue lysate. IHC-P: Mouse pituitary tissue. IHC-Fr: Mouse pituitary tissue. ICC/IF: GH3 cells. Flow Cyt (intra): GH3 cells. IP: Mouse pituitary tissue lysate. GH3 whole cell lysate.

  • 常规说明

    ab273057 is the carrier-free version of ab273048.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273057于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

靶标

  • 功能

    Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

  • 疾病相关

    Defects in POU1F1 are the cause of pituitary hormone deficiency combined type 1 (CPHD1) [MIM:613038]. CPHD is characterized by impaired production of growth hormone (GH) and one or more of the other five anterior pituitary hormones.

  • 序列相似性

    Belongs to the POU transcription factor family. Class-1 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Dwarf antibody
      • GHF 1 antibody
      • GHF 1A antibody
      • GHF-1 antibody
      • GHF1 antibody
      • GHF1A antibody
      • Growth hormone factor 1 antibody
      • Hmp 1 antibody
      • Hmp1 antibody
      • Pit 1 antibody
      • Pit 1 beta antibody
      • PIT 1Z antibody
      • PiT-1 antibody
      • Pit1 beta antibody
      • PIT1_HUMAN antibody
      • PIT1Z antibody
      • Pituitary growth hormone antibody
      • Pituitary specific positive transcription factor 1 antibody
      • Pituitary-specific positive transcription factor 1 antibody
      • POU class 1 homeobox 1 antibody
      • POU domain class 1 transcription factor 1 antibody
      • POU domain transcriptional regulator antibody
      • POU1F1 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Immunohistochemical analysis of paraffin-embedded mouse pituitary tissue labeling Pit1 with ab273048 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in mouse pituitary (PMID:21123951). Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pituitary tissue labeling Pit1 with ab273048 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). Nuclear staining on mouse pituitary is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Western blot - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Western blot - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      All lanes : Anti-Pit1 antibody [EPR23555-203] (ab273048) at 1/1000 dilution

      Lane 1 : Mouse pituitary tissue lysate
      Lane 2 : Mouse liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 33 kDa
      Observed band size: 33 kDa


      Exposure time: 81 seconds


      The expression profile observed is consistent with what has been described in the literature (PMID: 21123951).
      Negative control: Mouse liver (PMID: 21123951).

      Blocking/Diluting buffer and concentration 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Pit1 was immunoprecipitated from 0.35 mg mouse pituitary tissue lysate 10µg with ab273048 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273048 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse pituitary tissue lysate 10µg.

      Lane 2: ab273048 IP in mouse pituitary tissue lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273048 in mouse pituitary tissue lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 23 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized GH3 (Rat pituitary epithelial cell line) cells labelling Pit1 with ab273048 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2µg/ml dilution (Green). Confocal image showing nuclear staining in GH3 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2µg/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Flow Cytometry (Intracellular) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Flow Cytometry (Intracellular) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized GH3 (Rat pituitary epithelial cell line) cells labelling Pit1 with ab273048 at 1/50 dilution (1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048). 

       

       

    • Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Immunoprecipitation - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Pit1 was immunoprecipitated from 0.35 mg GH3 (Rat pituitary epithelial), whole cell lysate 10µg with ab273048 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273048 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: GH3 (Rat pituitary epithelial), whole cell lysate 10µg.

      Lane 2: ab273048 IP in GH3 whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab273048 in GH3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Immunohistochemistry (Frozen sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling Pit1 with ab273048 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution (Green). No staining on mouse liver is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2µg/ml) dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

      Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Pit1 with ab273048 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Counterstained with Hematoxylin.

      Negative control: No staining in mouse cerebrum (PMID:21123951).

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273048).

    • Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)
      Anti-Pit1 antibody [EPR23555-203] - BSA and Azide free (ab273057)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273057？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273057 尚未被引用在任何文献中。

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