AB10623

Anti-Pit1抗体[2C11]

Name: Pit1抗体[2C11] (ab10623)| Abcam中文官网
Brand: Abcam Limited
SKU: AB10623
Price: 4606 CNY
Availability: InStock

Anti-Pit1 antibody [2C11]

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(4 Publications)

Mouse Monoclonal PIT1 antibody. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Pou1f1 aa 1-150.

查看别名

Ghf-1, Pit-1, Pit1, Pou1f1, Pituitary-specific positive transcription factor 1, PIT-1, Growth hormone factor 1, GHF-1

1 Images

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Western blot - Anti-Pit1 antibody [2C11] (AB10623)

At 1 : 40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

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Western blot - Anti-Pit1 antibody [2C11] (ab10623)

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Western blot - Anti-Pit1 antibody [5E4] (<a href='/products/primary-antibodies/pit1-antibody-5e4-ab10545'>ab10545</a>)

Predicted band size: 33 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2C11

亚型

IgG1

轻链类型

kappa

不含载体蛋白

反应种属

Rat

应用

applications

免疫原

Recombinant Fragment Protein within Rat Pou1f1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P10037

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Pit1 also known as POU1F1 is a transcription factor with a mass of approximately 33 kDa. This protein is involved in the regulation of pituitary-specific genes and is critical for the development and function of the pituitary gland. It expresses mainly in the anterior pituitary where it activates several genes required for pituitary hormone production. Pit1 plays a role in differentiating somatotrophs lactotrophs and thyrotrophs which produce growth hormone prolactin and thyroid-stimulating hormone respectively.

Biological function summary

The Pit1 protein regulates the transcription of genes related to hormonal production in the anterior pituitary gland. It interacts with other transcription factors to form a complex that ensures the precise regulation of target genes. This regulation is essential for normal growth metabolism and reproductive functions. By controlling the expression of growth hormone thyroid-stimulating hormone and prolactin Pit1 directly affects various physiological processes in the body.

Pathways

Pit1 plays a vital role in the growth hormone (GH) and prolactin signaling pathways. It interacts with various regulatory proteins that modulate gene expression within these pathways. In the GH pathway Pit1 collaborates with proteins like GH-releasing hormone receptor to ensure adequate hormone levels in circulation. Additionally within the prolactin pathway Pit1 ensures the proper function of Jak-STAT signaling important for reproductive and metabolic functions. Its activity impacts important pathway components and further affects energy balance and cell proliferation.

Mutations or alterations in the Pit1 gene can lead to combined pituitary hormone deficiency (CPHD). This condition results in impaired hormone production affecting growth and development. Pit1 interacts with proteins like PROP1 which influences pituitary function and contributes to the pathogenesis of CPHD. Another disorder associated with Pit1 is congenital hypothyroidism where thyroid-stimulating hormone production is hindered impacting metabolism and growth. Understanding the role of Pit1 in these conditions can help in developing targeted therapies to manage hormonal disorders effectively.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

See full target information Pou1f1

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 160:2673-2691 PubMed31436800

2019

Decreased Activity of the Ghrhr and Gh Promoters Causes Dominantly Inherited GH Deficiency in Humanized GH1 Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Ariyasu,Emika Kubo,Daisuke Higa,Shinsuke Shibata,Yutaka Takaoka,Michihiko Sugimoto,Kazunori Imaizumi,Tomonobu Hasegawa,Kimi Araki

The Journal of biological chemistry 285:34718-28 PubMed20807761

2010

MicroRNAs regulate pituitary development, and microRNA 26b specifically targets lymphoid enhancer factor 1 (Lef-1), which modulates pituitary transcription factor 1 (Pit-1) expression.

Applications

IHC-P

Species

Mouse

Zichao Zhang,Sergio Florez,Arthur Gutierrez-Hartmann,James F Martin,Brad A Amendt

Journal of cellular biochemistry 92:664-78 PubMed15211565

2004

Inactivating Pit-1 mutations alter subnuclear dynamics suggesting a protein misfolding and nuclear stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Z Dave Sharp,David L Stenoien,Maureen G Mancini,Ilia I Ouspenski,Michael A Mancini

Journal of cellular biochemistry 72:322-38 PubMed10022514

1999

Subnuclear partitioning and functional regulation of the Pit-1 transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

M G Mancini,B Liu,Z D Sharp,M A Mancini

View all publications

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Anti-Pit1抗体[2C11]

Anti-Pit1 antibody [2C11]

Western blot - Anti-Pit1 antibody [2C11] (AB10623)

关键信息

宿主种属

克隆

克隆号

亚型

轻链类型

不含载体蛋白

反应种属

应用

免疫原