Anti-PIP5K3/PIKFYVE (phospho S307)抗体(ab62467)
Key features and details
- Rabbit polyclonal to PIP5K3/PIKFYVE (phospho S307)
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PIP5K3/PIKFYVE (phospho S307)抗体
参阅全部 PIP5K3/PIKFYVE 一抗 -
描述
兔多克隆抗体to PIP5K3/PIKFYVE (phospho S307) -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human PIP5K3/PIKFYVE aa 250-350 (phospho S307).
Database link: Q9Y2I7 -
阳性对照
- Human brain tissue and HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab62467 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62467于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/500 - 1/1000.
|
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| IHC-P |
1/50 - 1/100.
|
| 说明 |
|---|
|
ICC/IF
1/500 - 1/1000. |
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IHC-P
1/50 - 1/100. |
靶标
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功能
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes. -
疾病相关
Corneal dystrophy, fleck -
序列相似性
Contains 1 DEP domain.
Contains 1 FYVE-type zinc finger.
Contains 1 PIPK domain. -
细胞定位
Endosome membrane. Mainly associated with membranes of the late endocytic pathway. - Information by UniProt
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数据库链接
- Entrez Gene: 200576 Human
- Entrez Gene: 18711 Mouse
- Omim: 609414 Human
- SwissProt: Q9Y2I7 Human
- SwissProt: Q9Z1T6 Mouse
- Unigene: 744997 Human
- Unigene: 38370 Mouse
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别名
- 1 phosphatidylinositol 3 phosphate 5 kinase antibody
- 1 phosphatidylinositol 4 phosphate 5 kinase antibody
- 1-phosphatidylinositol 3-phosphate 5-kinase antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIP5K3/PIKFYVE (phospho S307) antibody (ab62467)
ab62467, at 1/50 dilution, staining PIP5K3/PIKFYVE in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-PIP5K3/PIKFYVE (phospho S307) antibody (ab62467)ab62467, at 1/500 dilution, staining PIP5K3/PIKFYVE in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab62467 被引用在 1 文献中.
- Mei S et al. Disruption of PIKFYVE causes congenital cataract in human and zebrafish. Elife 11:N/A (2022). PubMed: 35023829