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Anti-PIP2抗体[KT10] (ab2335)

Submit a review Q&A (6)References (11)

Key features and details

  • Mouse monoclonal [KT10] to PIP2
  • Suitable for: ICC/IF
  • Isotype: IgG2b

概述

  • 产品名称

    Anti-PIP2抗体[KT10]
    参阅全部 PIP2 一抗

  • 描述

    小鼠单克隆抗体[KT10] to PIP2

  • 宿主

    Mouse

  • 特异性

    This antibody cross reacts with Phosphatidic acid (<5.0%).

  • 经测试应用

    适用于: ICC/IFmore details

  • 免疫原

    Full length native protein (purified) corresponding to Bovine PIP2. Native Phosphatidylinositol-4,5-biphosphate (PIP2) from bovine spinal cord

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2335于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
Use at an assay dependent concentration.

TBS buffer is ideal.
说明
ICC/IF
Use at an assay dependent concentration.

TBS buffer is ideal.

靶标

  • 相关性

    Phosphatidylinositol 4,5-biphosphate (PIP2) is a membrane phospholipid that has been implicated in a variety of cellular processes, including synaptic vesicle recycling and signal transduction pathways. PLCD4 hydrolyzes PIP2 to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3).

文献 (11)

发表研究结果有使用 ab2335？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2335 被引用在 11 文献中.

  • Ray AT  et al. FGF signaling regulates development by processes beyond canonical pathways. Genes Dev 34:1735-1752 (2020). PubMed: 33184218
  • Assil S  et al. Plasmacytoid Dendritic Cells and Infected Cells Form an Interferogenic Synapse Required for Antiviral Responses. Cell Host Microbe 25:730-745.e6 (2019). PubMed: 31003939
  • Suzuki H  et al. Regulation of MC3T3-E1 differentiation by actin cytoskeleton through lipid mediators reflecting the cell differentiation stage. Biochem Biophys Res Commun 514:393-400 (2019). PubMed: 31047639
  • Roman-Garcia S  et al. Distinct Roles for Bruton's Tyrosine Kinase in B Cell Immune Synapse Formation. Front Immunol 9:2027 (2018). PubMed: 30237801
  • Mkrtschjan MA  et al. Lipid signaling affects primary fibroblast collective migration and anchorage in response to stiffness and microtopography. J Cell Physiol 233:3672-3683 (2018). PubMed: 29034471
View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Question


I am wondering if ab2335 is the same clone (KT 10) as was developed by Fukami, et al in the 1988 PNAS paper (Vol. 85 pp.9057-61). If not, any information about the development of this specific clone would be much appreciated.
I am trying to look for PIP2 in Megakaryocytes (hematopoeitic cells) and am looking for a good ab for IF.

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Answer

Thank you for contacting Abcam

I have talked to the supplier of this antibody and I am sorry but they do not have any detailed clone information that I can share and cannot confirm that this antibody is the same as the one published in the paper indicated.
Sorry that I cannot be of more help in this matter, but if there is anything else I can help you with, please elt me know.

Free RabMab with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Question

Does this antibody cross-react with PIP3? What protocols were used to test this antibody in ELISA and ICC?

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Answer

Thank you very much for your call last week, and I'm very sorry for the delay getting back to you.

I've just heard from the lab, and unfortunately ab2335 has not been tested for cross-reactivity with PIP3. The original clone was produced and tested by an outside lab, and we don't have the full ELISA or ICC protocols at this time.

I apologize that we don't have more information about this antibody, but if you have further questions or comments, or if there is anything else that we can do for you, please let me know and I'll be happy to help.

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Question

What is the cross reactivity of this antibody with other PtdIns?

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Answer

Thank you for your enquiry. The cross reactivity of this antibody with other PtdIns is stated below. PIP2 100%; Phosphatidic acid <5.0%; PIP 0.1%; Phosphatidyl inositol <0.2%; Phosphatidyl choline <0.2%; Phosphatidyl serine <0.2%; Phosphatidyl ethanolamine <0.2%; Phosphatidyl glycerol <0.2%; Cardiolipin <0.2%; Cholesterol <0.2%; Diacylglycerol <0.2%

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Question

Is ab2335 clone 2C11?

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Answer

Thank you for your enquiry. No ab2335 is not clone 2C11. Please do not hesitate to contact me should you require further assistance.

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Question

Has ab2335 been tested yet for immunocytochemistry?

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Answer

To our knowledge, this antibody has yet to be tested in ICC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. If you have any additional questions, please contact us again.

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Question

According to your datasheet, ab2335's tested applications are ELISA and radioimmunoassay. Is there a published ELISA potocol using ab2335? If so, what is the reference? Also, is there information on the cross reactivity to other phosphatidylinositols? regards,

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Answer

Thank you for your enquiry, Here are two publications citing the use of this antibody: RIA: Fukami, K., Takenawa, T. (1989) Quantitative Changes in Polysphophoinositides 1,2-diacyglycerol and Inositol 1,4,5-Trisphosphate by Platelet-Derived Growth Factor and Prostaglandin F2a. The Journal of Biological Chemistry 264(25):1485-1489. ELISA: Imai, K., et al. (1999) The Pleckstrin Homology Domain of the Wiskott-Aldrich Syndrome Protein Is Involved in the Organization of Actin Cytoskeleton. Clinical Immunology 92(2):128-137. We do not have any information regarding the cross reactivity to other phosphatidylinositols, my apologies for the inconvenience,

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