Anti-PINCH-2抗体(ab272666)
Key features and details
- Rabbit polyclonal to PINCH-2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PINCH-2抗体 -
描述
兔多克隆抗体to PINCH-2 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human PINCH-2 aa 300-400.
Database link: Q7Z4I7 -
阳性对照
- WB: Human liver and tonsil tissue. IHC-P: Human prostate, kidney and skin tissue. IF: BJ cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272666于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Competes with LIMS1 for binding to ILK. Plays a role in modulating cell spreading and migration. -
序列相似性
Contains 5 LIM zinc-binding domains. -
细胞定位
Nucleus. Cell junction > focal adhesion. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 55679 Human
- Omim: 607908 Human
- SwissProt: Q7Z4I7 Human
- Unigene: 469881 Human
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别名
- FLJ10044 antibody
- ILK binding protein antibody
- LIM and senescent cell antigen like domains 2 antibody
see all
图片
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Western blot - Anti-PINCH-2 antibody (ab272666)Lanes 2-6 : Anti-PINCH-2 antibody (ab272666) at 0.4 µg/ml
Lane 1 : Molecular Weight ladder
Lane 2 : RT4 cells (Human urinary bladder cancer cell line)
Lane 3 : U-251 MG cells (Human brain glioma cell line)
Lane 4 : Human plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue
Lane 6 : Human tonsil tissue -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human prostate tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-PINCH-2 antibody (ab272666)PFA fixed, Triton X-100 permeablized BJ cells (human skin fibroblast cell line) labeling PINCH-2 (green) using ab272666 at 2 ug/ml in ICC/IF analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human kidney tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human skin tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH-2 antibody (ab272666)Paraffin-embedded formalin-fixed human pancreas tissue stained for PINCH-2 using ab272666 at 1/500 dilution in immunohistochemical analysis. Shows low expression as expected.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab272666 被引用在 2 文献中.
- Su C et al. LIMS2 is Downregulated in Osteosarcoma and Inhibits Cell Growth and Migration. J Oncol 2022:4811260 (2022). PubMed: 36276291
- Wang J & Zhang S Fluid shear stress modulates endothelial inflammation by targeting LIMS2. Exp Biol Med (Maywood) 245:1656-1663 (2020). PubMed: 32752897