重组Anti-PINCH 1 + PINCH 2抗体[EP1943Y] (ab76112)
![Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)](https://www.abcam.cn/ps/products/76/ab76112/Images/ab76112-519040-anti-pinch-1-pinch-2-antibody-ep1943y-western-blot-human-hela-mouse-kidney.jpg "Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1943Y] to PINCH 1 + PINCH 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PINCH 1 + PINCH 2抗体[EP1943Y] -
描述
兔单克隆抗体[EP1943Y] to PINCH 1 + PINCH 2 -
宿主
Rabbit -
特异性
The immunogen used for this product shares 73% homology with PINCH 2. Cross-reactivity with this protein has not been confirmed experimentally.
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经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: His-tagged Human full length recombinant LIMS1 and LIMS2 proteins, HeLa, Jurkat, HEK-293, and Mouse and Rat kidney lysate.
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常规说明
This product has switched from a hybridoma to recombinant production method on 7th September 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1943Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76112于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000.
|
| 说明 |
|---|
|
WB
1/1000 - 1/5000. |
靶标
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细胞定位
PINCH 1: Cell junction > focal adhesion. Cell membrane. PINCH-2: Nucleus. Cell junction > focal adhesion. Cell membrane. -
数据库链接
- Entrez Gene: 3987 Human
- Entrez Gene: 55679 Human
- Entrez Gene: 110829 Mouse
- Entrez Gene: 225341 Mouse
- Entrez Gene: 361303 Rat
- Entrez Gene: 499443 Rat
- Omim: 602567 Human
- Omim: 607908 Human
see all
图片
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Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/5000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate
Lane 2 : Mouse kidney lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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![Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)](https://www.abcam.cn/ps/products/76/ab76112/Images/ab76112-519041-anti-pinch-1-pinch-2-antibody-ep1943y-western-blot-human-jurkat-hek-293-rat-kidney.jpg)
Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole lysate
Lane 3 : Rat kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 34 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
-
![Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)](https://www.abcam.cn/ps/products/76/ab76112/Images/ab76112-519037-anti-pinch-1-and-pinch-2-antibody-ep1943y-western-blot.jpg)
Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112)All lanes : Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution
Lane 1 : His-tagged Human full length recombinant LIMS1 protein, 20 ng
Lane 2 : His-tagged Human full length recombinant LIMS2 protein, 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 34 and 37 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab76112 被引用在 1 文献中.
- Draicchio F et al. Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance haemodialysis patients. J Physiol 598:5701-5716 (2020). PubMed: 32969494