AB198842

Anti-PIM3抗体- C-terminal

Name: PIM3抗体- C-terminal (ab198842)| Abcam中文官网
Brand: Abcam Limited
SKU: AB198842
Price: 4973 CNY
Availability: InStock

Anti-PIM3 antibody - C-terminal

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(4 Publications)

Rabbit Polyclonal PIM3 antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human PIM3.

查看别名

Serine/threonine-protein kinase pim-3, PIM3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM3 antibody - C-terminal (AB198842)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM3 antibody - C-terminal (AB198842)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PIM3 using ab198842 at 1/20

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM3 antibody - C-terminal (AB198842)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using ab198842 at 1/50 dilution, (A) without and (B) with synthetic peptide treatment.

Supplier Data

Western blot - Anti-PIM3 antibody - C-terminal (AB198842)

Primary antibody was incubated overnight at 4˚C.

All lanes:

Western blot - Anti-PIM3 antibody - C-terminal (ab198842) at 1/1000 dilution

All lanes:

HeLa cell lysate at 40 µg

Secondary

All lanes:

HRP-conjugated Goat anti rabbit IgG at 1/5000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

false

Supplier Data

Western blot - Anti-PIM3 antibody - C-terminal (AB198842)

All lanes:

Western blot - Anti-PIM3 antibody - C-terminal (ab198842) at 1/250 dilution

All lanes:

Mouse skeletal muscle tissue lysate at 40 µg

Predicted band size: 36 kDa

false

Exposure time: 5min

Supplier Data

Western blot - Anti-PIM3 antibody - C-terminal (AB198842)

All lanes:

Western blot - Anti-PIM3 antibody - C-terminal (ab198842) at 1/1350 dilution

All lanes:

Mouse brain tissue lysate at 40 µg

Predicted band size: 36 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

IHC-P, WB

applications

免疫原

Synthetic Peptide within Human PIM3. The exact immunogen used to generate this antibody is proprietary information.

Q86V86

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/25 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The PIM3 protein is a member of the proviral integration site for moloney murine leukemia virus (PIM) serine/threonine kinase family. It is also known as PIM-3 kinase. This protein has a mass of approximately 37 kDa. Expression of PIM3 is found in various tissues including liver pancreas kidney and some cancer cell lines. Scientists recognize its role in phosphorylating target proteins which affects various cellular processes like cell cycle and apoptosis.

Biological function summary

PIM3 functions by regulating cell survival and proliferation. It does not form part of any large protein complex but operates through phosphorylation of substrates. This kinase modulates signaling pathways important for mitogenic stimuli and cellular responses to various stress factors. Tissues with abnormal PIM3 levels may experience altered cell growth and survival which can affect normal physiological processes.

Pathways

PIM3 interacts with cellular signaling networks. It is notably involved in the PI3K/AKT signaling pathway affecting cell growth and metabolism. Additionally PIM3 participates in mTOR signaling linking it to protein synthesis and cellular energy balance. It shows functional relation with proteins such as AKT and mTOR suggesting its integration in these pivotal regulatory circuits.

Excessive PIM3 expression associates with certain types of cancer such as pancreatic cancer and hepatocellular carcinoma. It contributes to oncogenic processes by promoting uncontrolled cell growth and survival. In cancer contexts PIM3 interacts with oncogenes like c-MYC which may exacerbate tumorous characteristics. Targeting PIM3 pathways offers potential therapeutic strategies for related malignancies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through : the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.

See full target information PIM3

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 14:1483660 PubMed39507762

2024

Low miR-936-mediated upregulation of Pim-3 drives sorafenib resistance in liver cancer through ferroptosis inhibition by activating the ANKRD18A/Src/NRF2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Li,Mengna Cui,Long Xu,Qie Guo

World journal of surgical oncology 21:76 PubMed36871027

2023

Role and mechanism of PIM family in the immune microenvironment of diffuse large B cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Changying Wang,Qitian Chen,Haichao Luo,Ran Chen

Journal of cellular and molecular medicine 24:12840-12847 PubMed32945069

2020

Inhibitory effect of Ubenimex combined with fluorouracil on multiple drug resistance and P-glycoprotein expression level in non-small lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wan,Xie-An Ling,Jian Wang,Guang-Gui Ding,Xi Wang

BMC cancer 20:571 PubMed32552717

2020

Suppression of Mig-6 overcomes the acquired EGFR-TKI resistance of lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Da Hyun Kang,Sung Soo Jung,Min-Kyung Yeo,Da Hye Lee,Geon Yoo,Sang Yeon Cho,In-Jae Oh,Ju-Ock Kim,Hee Sun Park,Chaeuk Chung,Jeong Eun Lee

View all publications

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Anti-PIM3抗体- C-terminal

Anti-PIM3 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM3 antibody - C-terminal (AB198842)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM3 antibody - C-terminal (AB198842)

Western blot - Anti-PIM3 antibody - C-terminal (AB198842)

Western blot - Anti-PIM3 antibody - C-terminal (AB198842)

Western blot - Anti-PIM3 antibody - C-terminal (AB198842)