重组Anti-PIM2抗体[EPR6987] (ab129057)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6987] to PIM2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PIM2抗体[EPR6987]
参阅全部 PIM2 一抗 -
描述
兔单克隆抗体[EPR6987] to PIM2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PIM2 aa 1-100 (N terminal). The exact sequence is proprietary.
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阳性对照
- WB: Jurkat, K562, Raji, HL60 and 293T cell lysates, Human heart, Mouse heart, Rat heart, Human kidney, and Mouse kidney tissue lysates; IP: K-562 cell lysate
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.88 x 10 -10 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6987 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129057于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 34-40 kDa (predicted molecular weight: 34 kDa).
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 34-40 kDa (predicted molecular weight: 34 kDa). |
IP
1/10 - 1/100. |
靶标
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功能
Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappaB kinase/NF-kappaB cascade; this process requires phosphorylation of MAP3K8/COT. Prevents apoptosis induced by growth factor withdrawal via inhibition of caspase-3 activation, and via phosphorylation of pro-apoptotic proteins. Inhibits BAD-induced cell death via phosphorylation of BAD. PIM2-mediated cell survival is glucose-dependent but independent of several AKT regulators such as PI3K, HSP-90 and TOR, indicating that PIM2 and PI3K/AKT/TOR function via distinct pathways. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate. -
组织特异性
Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated. - Information by UniProt
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数据库链接
- Entrez Gene: 11040 Human
- Entrez Gene: 18715 Mouse
- Entrez Gene: 317366 Rat
- Omim: 300295 Human
- SwissProt: Q9P1W9 Human
- SwissProt: Q62070 Mouse
- Unigene: 726382 Human
- Unigene: 347478 Mouse
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别名
- OTTHUMP00000025825 antibody
- Pim 2 oncogene antibody
- Pim 2h antibody
see all
图片
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All lanes : Anti-PIM2 antibody [EPR6987] (ab129057) at 1/10000 dilution (Purified)
Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
Lane 2 : Human heart lysates
Lane 3 : Mouse heart lysates
Lane 4 : Rat heart lysates
Lane 5 : Human kidney lysates
Lane 6 : Mouse kidney lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 34-44 kDa why is the actual band size different from the predicted?The multiple bands are consistent with what has been described in PMID: 7781606 and 24659821
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ab129057 (purified) at 1:100 dilution (2µg) immunoprecipitating PIM2 in K-562 whole cell lysate.
Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
Lane 2 (+): ab129057 & K-562 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129057 in K-562 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-PIM2 antibody [EPR6987] (ab129057) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HL60 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 34 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab129057 被引用在 4 文献中.
- Ma S et al. An XBP1s-PIM-2 positive feedback loop controls IL-15-mediated survival of natural killer cells. Sci Immunol 8:eabn7993 (2023). PubMed: 36897958
- Ding J et al. Role of PIM2 in acute lung injury induced by sepsis. Exp Ther Med 24:543 (2022). PubMed: 35978927
- Su D et al. PTIP chromatin regulator controls development and activation of B cell subsets to license humoral immunity in mice. Proc Natl Acad Sci U S A 114:E9328-E9337 (2017). Flow Cyt ; Mouse . PubMed: 29078319
- Horiuchi D et al. PIM1 kinase inhibition as a targeted therapy against triple-negative breast tumors with elevated MYC expression. Nat Med 22:1321-1329 (2016). PubMed: 27775705