AB129057

重组Anti-PIM2抗体[EPR6987]

Anti-PIM2 antibody [EPR6987]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Recombinant Monoclonal PIM2 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

查看别名

Serine/threonine-protein kinase pim-2, Pim-2h, PIM2

4 Images

Unknown

Immunoprecipitation - Anti-PIM2 antibody [EPR6987] (AB129057)

ab129057 (purified) at 1 : 100 dilution (2μg) immunoprecipitating PIM2 in K-562 whole cell lysate.
Lane 1 (input) : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10μg
Lane 2 (+) : ab129057 & K-562 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab129057 in K-562 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-PIM2 antibody [EPR6987] (ab129057)

Predicted band size: 34 kDa

false

Lab

Western blot - Anti-PIM2 antibody [EPR6987] (AB129057)

The multiple bands are consistent with what has been described in PMID : 7781606 and 24659821

All lanes:

Western blot - Anti-PIM2 antibody [EPR6987] (ab129057) at 1/10000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Lane 2:

Human heart lysates at 20 µg

Lane 3:

Mouse heart lysates at 20 µg

Lane 4:

Rat heart lysates at 20 µg

Lane 5:

Human kidney lysates at 20 µg

Lane 6:

Mouse kidney lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 34 kDa

Observed band size: 34-44 kDa

false

Unknown

Western blot - Anti-PIM2 antibody [EPR6987] (AB129057)

All lanes:

Western blot - Anti-PIM2 antibody [EPR6987] (ab129057) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Raji cell lysate at 10 µg

Lane 4:

HL60 cell lysate at 10 µg

Lane 5:

293T cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 34 kDa

false

OI-RD Scanning - Anti-PIM2 antibody [EPR6987] (AB129057)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-PIM2 antibody [EPR6987] (AB129057)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-PIM2 antibody [EPR6987] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR6987

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PIM2 also known as Pim-2 proto-oncogene encodes a serine/threonine kinase with a molecular weight of approximately 34 kDa. This protein is expressed in various tissues with higher levels in hematopoietic and lymphoid systems. PIM2 kinase activity increases under certain cellular stress conditions and it functions by phosphorylating substrates involved in cell survival proliferation and apoptosis regulation. As a member of the PIM kinase family it shares functional characteristics with its relatives PIM1 and PIM3.

Biological function summary

The actions of PIM2 influence cell cycle progression and survival. It does not form a complex but interacts with several other proteins. PIM2 phosphorylates important substrates such as BAD and 4E-BP1 which impacts the cellular translation machinery and apoptotic pathways. Through these interactions PIM2 contributes to cellular responses to growth factors and various stress signals promoting cell survival even in low nutrient or hypoxic conditions.

Pathways

In the PI3K/AKT and MYC signaling pathways PIM2 has important roles. PIM2 contributes by regulating the stability and activity of MYC a transcription factor vital for cancer cell metabolism. In the PI3K/AKT pathway PIM2 activity works synergistically with AKT to enhance pro-survival and proliferative signals. These actions link PIM2 with both cellular growth and survival pathways often cooperating with proteins like mTOR and BAD to modulate downstream effects.

Aberrant PIM2 expression has been associated with several malignancies including leukemia and multiple myeloma. The elevated PIM2 levels in cancer cells often correlate with resistance to apoptosis contributing to tumor progression. In these contexts PIM2 interacts with oncogenic proteins such as MYC and mTOR establishing a network that supports the survival and growth of tumor cells. Targeting PIM2 directly or its associated pathways offers potential therapeutic strategies for these cancers.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through : the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.

See full target information PIM2

style

left-column

文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Communications medicine 4:22 PubMed38378783

2024

S100A8/A9 predicts response to PIM kinase and PD-1/PD-L1 inhibition in triple-negative breast cancer mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren R Begg,Adrienne M Orriols,Markella Zannikou,Chen Yeh,Pranathi Vadlamani,Deepak Kanojia,Rosemary Bolin,Sara F Dunne,Sanjeev Balakrishnan,Roman Camarda,Diane Roth,Nicolette A Zielinski-Mozny,Christina Yau,Athanassios Vassilopoulos,Tzu-Hsuan Huang,Kwang-Youn A Kim,Dai Horiuchi

Science immunology 8:eabn7993 PubMed36897958

2023

An XBP1s-PIM-2 positive feedback loop controls IL-15-mediated survival of natural killer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shoubao Ma,Jingjing Han,Zhenlong Li,Sai Xiao,Jianying Zhang,Jiazhuo Yan,Tingting Tang,Tasha Barr,Andrew S Kraft,Michael A Caligiuri,Jianhua Yu

Experimental and therapeutic medicine 24:543 PubMed35978927

2022

Role of PIM2 in acute lung injury induced by sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Juncai Ding,Xiufang Yang,Huijuan Huang,Bo Wang

Proceedings of the National Academy of Sciences of 114:E9328-E9337 PubMed29078319

2017

PTIP chromatin regulator controls development and activation of B cell subsets to license humoral immunity in mice.

Applications

Flow Cyt

Species

Mouse

Dan Su,Stijn Vanhee,Rebeca Soria,Elin Jaensson Gyllenbäck,Linda M Starnes,Martina Kubec Højfeldt,Gabriel K Pedersen,Joan Yuan,Jeremy A Daniel

Nature medicine 22:1321-1329 PubMed27775705

2016

PIM1 kinase inhibition as a targeted therapy against triple-negative breast tumors with elevated MYC expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dai Horiuchi,Roman Camarda,Alicia Y Zhou,Christina Yau,Olga Momcilovic,Sanjeev Balakrishnan,Alexandra N Corella,Henok Eyob,Kai Kessenbrock,Devon A Lawson,Lindsey A Marsh,Brittany N Anderton,Julia Rohrberg,Ratika Kunder,Alexey V Bazarov,Paul Yaswen,Michael T McManus,Hope S Rugo,Zena Werb,Andrei Goga

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-PIM2抗体[EPR6987]