重组Anti-PIM1抗体[S08-8D5] (ab317411)
![Western blot - Anti-PIM1 Antibody [S08-8D5] (ab317411)](https://www.abcam.cn/ps/products/317/ab317411/Images/ab317411-1-anti-pim1-antibody-s08-8d5-western-blot-hela-a549-hl60-human.jpg "Western blot - Anti-PIM1 Antibody [S08-8D5] (ab317411)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [S08-8D5] to PIM1
- Suitable for: WB, IHC-P
- Reacts with: Human
概述
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产品名称
Anti-PIM1抗体[S08-8D5]
参阅全部 PIM1 一抗 -
描述
兔单克隆抗体[S08-8D5] to PIM1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human PIM1.
Database link: P11309 -
阳性对照
- WB: HeLa, A549 and HL-60 cell lysate. IHC-P: Human tonsil tissue.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA -
Concentration information loading... -
纯度
Affinity purified -
克隆
单克隆 -
克隆编号
S08-8D5 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317411于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 45 kDa.
|
|
| IHC-P |
1/20 - 1/50.
|
| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 45 kDa. |
|
IHC-P
1/20 - 1/50. |
靶标
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功能
May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. Isoform 2 promotes the G1/S transition of the cell cycle via up-regulation of CDK2 activity and phosphorylation of CDKN1B, resulting in enhanced nuclear export and proteasome-dependent degradation of CDKN1B. Isoform 2 also represses CDKN1B transcription by phosphorylating and inactivating the transcription factor FOXO3. Plays a role in signal transduction in blood cells. Contributes to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. -
组织特异性
Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated on both serine/threonine and tyrosine residues. -
细胞定位
Cytoplasm. Nucleus and Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 5292 Human
- Omim: 164960 Human
- SwissProt: P11309 Human
- Unigene: 81170 Human
-
别名
- Oncogene PIM 1 antibody
- Oncogene PIM1 antibody
- PIM 1 antibody
see all
图片
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Western blot - Anti-PIM1 Antibody [S08-8D5] (ab317411)All lanes : Anti-PIM1 Antibody [S08-8D5] (ab317411) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HL-60 cell lysate
Predicted band size: 45 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM1 Antibody [S08-8D5] (ab317411)](https://www.abcam.cn/ps/products/317/ab317411/Images/ab317411-536859-anti-pim1-antibody-s08-8d5-immunohistochemistry-tonsil-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIM1 Antibody [S08-8D5] (ab317411)Immunohistochemistry of PIM1 in paraffin-embedded human tonsil using ab317411 at dilution 1/50.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317411 尚未被引用在任何文献中。