AB224118

Anti-PIKE抗体

Name: PIKE抗体 (ab224118)| Abcam中文官网
Brand: Abcam Limited
SKU: AB224118
Price: 5757 CNY
Availability: InStock

Anti-PIKE antibody

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(2 Publications)

Rabbit Polyclonal PIKE antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AGAP2 aa 700-850.

查看别名

CENTG1, KIAA0167, AGAP2, AGAP-2, Centaurin-gamma-1, GTP-binding and GTPase-activating protein 2, Phosphatidylinositol 3-kinase enhancer, Cnt-g1, GGAP2, PIKE

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PIKE antibody (AB224118)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PIKE antibody (AB224118)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PIKE (green) using ab224118 (4 μg/ml) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIKE antibody (AB224118)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIKE antibody (AB224118)

Paraffin-embedded human cerebellum tissue stained for PIKE with ab224118 (1/50) in immunohistochemical analysis.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human AGAP2 aa 700-850. The exact immunogen used to generate this antibody is proprietary information.

Q99490

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PIKE also known as phosphoinositide 3-kinase enhancer is a protein encoded by the CENTG1 gene. It weighs approximately 97 kDa and has several isoforms including PIKE-A PIKE-L and PIKE-S each with distinct localizations and functions. PIKE is expressed in neural tissues especially in the brain where it regulates numerous signaling processes. This protein facilitates the activation of phosphoinositide 3-kinase (PI3K) by directly interacting with it thereby enhancing downstream signaling.

Biological function summary

Proteins like PIKE are important in regulating cellular growth survival and proliferation. PIKE acts as an important mediator in the PI3K signaling pathway and is known to affect neuronal cell development and neurite outgrowth. It participates in forming complexes with other proteins such as Akt further amplifying signaling cascades linked to cell survival and growth. Its prominent role in neural systems makes PIKE significant in maintaining normal brain function.

Pathways

The protein PIKE integrates significantly into the PI3K/Akt signaling pathway and the small GTPase pathway. In the PI3K/Akt pathway PIKE works closely with Akt and mTOR proteins promoting cell survival and growth. In the small GTPase pathway PIKE interacts with proteins like Rac1 which regulates actin cytoskeleton dynamics and influences changes in cell shape and motility. These pathways highlight PIKE's involvement in essential cellular processes.

The abnormal regulation of PIKE links to specific conditions such as cancer and schizophrenia. In cancer altered PIKE activity contributes to tumorigenesis through its interaction with growth-related proteins including Akt. In schizophrenia changes in PIKE expression or function can affect neural signaling pathways potentially influencing cognitive and behavioral symptoms associated with the disorder. Understanding PIKE's role in these conditions could aid in developing new therapeutic strategies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.

See full target information AGAP2

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular gastroenterology and hepatology 15:533-558 PubMed36270602

2022

Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Yi Li,Kazuyuki Kuroki,Tetsuro Shimakami,Kazuhisa Murai,Kazunori Kawaguchi,Takayoshi Shirasaki,Kouki Nio,Saiho Sugimoto,Tomoki Nishikawa,Hikari Okada,Noriaki Orita,Hideo Takayama,Ying Wang,Phuong Doan Thi Bich,Astuya Ishida,Sadahiro Iwabuchi,Shinichi Hashimoto,Takeshi Shimaoka,Noriko Tabata,Miho Watanabe-Takahashi,Kiyotaka Nishikawa,Hiroshi Yanagawa,Motoharu Seiki,Kouji Matsushima,Taro Yamashita,Shuichi Kaneko,Masao Honda

Aging 11:5300-5318 PubMed31386624

2019

Exosomes derived from microRNA-199a-overexpressing mesenchymal stem cells inhibit glioma progression by down-regulating AGAP2.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yu,Si Gui,Yawei Liu,Xiaoyu Qiu,Guozhong Zhang,Xi'an Zhang,Jun Pan,Jun Fan,Songtao Qi,Binghui Qiu

View all publications

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Anti-PIKE抗体

Anti-PIKE antibody

Immunocytochemistry/ Immunofluorescence - Anti-PIKE antibody (AB224118)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIKE antibody (AB224118)