AB133773

重组Anti-PICK1抗体[EPR4130(3)]

Anti-PICK1 antibody [EPR4130(3)]

RabMAb
Recombinant
KO Validated
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal PICK1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

PRKCABP, PICK1, PRKCA-binding protein, Protein interacting with C kinase 1, Protein kinase C-alpha-binding protein

3 Images

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Western blot - Anti-PICK1 antibody [EPR4130(3)] (AB133773)

All lanes:

Western blot - Anti-PICK1 antibody [EPR4130(3)] (ab133773) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

LnCaP lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

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Lab

Western blot - Anti-PICK1 antibody [EPR4130(3)] (AB133773)

Western blot : Rabbit Monoclonal[EPR4130(3)] to PICK1 ab133773 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 47 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in PICK1 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PICK1 antibody [EPR4130(3)] (ab133773) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

PICK1 knockout U-87 MG at 20 µg

Lane 3:

T-47D at 20 µg

Lane 4:

MCF7 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

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OI-RD Scanning - Anti-PICK1 antibody [EPR4130(3)] (AB133773)

OI-RD Scanning

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OI-RD Scanning - Anti-PICK1 antibody [EPR4130(3)] (AB133773)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

不同偶联物与剂型 (1)

Carrier free

Anti-PICK1 antibody [EPR4130(3)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4130(3)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯度

Tissue culture supernatant

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PICK1 or Protein Interacting with C Kinase 1 is a protein that plays a significant role in cellular processes. With a molecular mass of approximately 47 kDa PICK1 is well known for its ability to bind to proteins through its PDZ domain. You can find PICK1 expressed highly in the brain but it is also present in other tissues like the pancreas and testes. This makes it an important target for understanding various cellular interactions and functions related to protein trafficking.

Biological function summary

PICK1 is known for its role in modulating the trafficking and synaptic targeting of AMPA receptors which are essential for synaptic plasticity and memory formation. It operates as part of a protein complex that influences the receptor's presence at the synaptic membrane. PICK1 binds with proteins like GluA2 subunit of AMPA receptors which allows it to regulate receptor internalization and surface expression impacting synaptic signaling and plasticity.

Pathways

PICK1 interacts within the glutamatergic signaling and insulin secretion pathways. In the glutamatergic signaling pathway it contributes to the regulation of synaptic strength by linking with other proteins such as protein kinase C (PKC) to facilitate synapse dynamics. In insulin secretion PICK1 partners with proteins like the insulin granule docking machinery to regulate insulin release in response to glucose.

Research has linked PICK1 function with conditions like schizophrenia and diabetes. PICK1's regulatory influence on AMPA receptors involves interactions with proteins such as protein kinase C which researchers have associated with synaptic abnormalities seen in schizophrenia. Regarding diabetes PICK1's role in insulin secretion connects it to the disease through the regulation of proteins involved in insulin granule dynamics. Understanding these connections aids in developing potential therapeutic strategies for these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate ASIC1/ASIC3 channel. Regulates actin polymerization by inhibiting the actin-nucleating activity of the Arp2/3 complex; the function is competitive with nucleation promoting factors and is linked to neuronal morphology regulation and AMPA receptor (AMPAR) endocytosis. Via interaction with the Arp2/3 complex involved in regulation of synaptic plasicity of excitatory synapses and required for spine shrinkage during long-term depression (LTD). Involved in regulation of astrocyte morphology, antagonistic to Arp2/3 complex activator WASL/N-WASP function.

See full target information PICK1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 290:6387-96 PubMed25564616

2015

Rictor/mTORC2 pathway in oocytes regulates folliculogenesis, and its inactivation causes premature ovarian failure.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenguo Chen,Xiangjin Kang,Liping Wang,Heling Dong,Caixia Wang,Zhi Xiong,Wanlu Zhao,Chunhong Jia,Jun Lin,Wen Zhang,Weiping Yuan,Mei Zhong,Hongzi Du,Xiaochun Bai

View all publications

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重组Anti-PICK1抗体[EPR4130(3)]