AB72118

Anti-PIBF抗体

Name: PIBF抗体 (ab72118)| Abcam中文官网
Brand: Abcam Limited
SKU: AB72118
Price: 4606 CNY
Availability: InStock

Anti-PIBF antibody

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(3 Publications)

Rabbit Polyclonal PIBF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PIBF1 aa 700-800.

查看别名

C13orf24, PIBF, PIBF1, Progesterone-induced-blocking factor 1, Centrosomal protein of 90 kDa, CEP90

2 Images

Unknown

Immunoprecipitation - Anti-PIBF antibody (AB72118)

Detection of PIBF by Western Blot of Immunprecipitate.
ab72118 at 1 μg/ml staining PIBF in HeLa whole cell lysate immunoprecipitated using ab72118 at 3 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-PIBF antibody (ab72118)

Predicted band size: 90 kDa

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Unknown

Western blot - Anti-PIBF antibody (AB72118)

All lanes:

Western blot - Anti-PIBF antibody (ab72118) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 90 kDa

Observed band size: 90 kDa

true

Exposure time: 30s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human PIBF1 aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab72118 was affinity purified using an epitope specific to PIBF immobilized on solid support.

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The progesterone-induced blocking factor (PIBF) is a protein with a molecular mass of approximately 90 kDa. It has important roles linked to cell division and immune response regulation. PIBF is produced in larger quantities in pregnancy tissues particularly by decidual and trophoblastic cells but normal lymphocytes also express it when stimulated by progesterone. Researchers often study its activity because of its potential relevance in pregnancy and immune system interactions.

Biological function summary

Researchers already documented that PIBF plays a role in modulating immune responses during pregnancy. It acts as part of a complex involving progesterone and cytokines working to prevent the mother's immune system from rejecting the fetus. PIBF achieves this by influencing the Th1/Th2 cytokine balance increasing anti-inflammatory cytokines and decreasing pro-inflammatory cytokines like IL-6 and IL-12. This helps to maintain a tolerogenic environment during pregnancy.

Pathways

The PIBF significantly affects pathways involving immune regulation and inflammation. It interacts with the hormonal pathway of progesterone linking it to the immune-modulatory functions controlled by cytokine action. The balance between these pathways ensures immune tolerance in the placenta. PIBF also shares a functional pathway with proteins such as interleukin-10 (IL-10) as they both modulate immune responses to support successful pregnancies.

PIBF has significant associations with pregnancy-related conditions and certain cancers. Abnormal PIBF levels may relate to pregnancy complications such as recurrent spontaneous abortion where immune tolerance gets disrupted. Additionally PIBF can impact tumor growth because it promotes an immunosuppressive environment similar to that observed in pregnancy potentially benefiting malignant cells. This connection suggests that PIBF along with related proteins like IL-10 could serve as a target for therapeutic interventions in these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in ciliogenesis.. Isoform 1. Pericentriolar protein required to maintain mitotic spindle pole integrity (PubMed : 21224392). Required for the centrosomal accumulation of PCM1 and the recruitment of centriolar satellite proteins such as BBS4. Via association with PCM1 may be involved in primary cilia formation (PubMed : 23110211). Required for CEP63 centrosomal localization and its interaction with WDR62. Together with CEP63 promotes centriole duplication. Promotes the centrosomal localization of CDK2 (PubMed : 26297806).. Isoform 4. The secreted form is a mediator of progesterone that by acting on the phospholipase A2 enzyme interferes with arachidonic acid metabolism, induces a Th2 biased immune response, and by controlling decidual natural killer cells (NK) activity exerts an anti-abortive effect (PubMed : 12516630, PubMed : 14634107, PubMed : 3863495). Increases the production of Th2-type cytokines by signaling via the JAK/STAT pathway. Activates STAT6 and inhibits STAT4 phosphorylation. Signaling via a not identified receptor seems to implicate IL4R and a GPI-anchored protein (PubMed : 16393965, PubMed : 25218441).

See full target information PIBF1

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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:30658 PubMed40835624

2025

PIBF1 expression and survival outcome in TNBC and Non-TNBC breast cancer patients with lymph node metastasis who undertaken chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Eunju Shin,Jewon Ryu,Tae-Kyung Yoo,Sae Byul Lee,Jisun Kim,Il Yong Chung,Beom Seok Ko,Hee Jeong Kim,Jong Won Lee,Jun Hyeong Lee,Kyunggon Kim,Sang-Wook Lee,Byung Ho Son

Scientific reports 14:13989 PubMed38886371

2024

In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Jado,Michelle Dow,Krypton Carolino,Adam Klie,Gregory J Fonseca,Trey Ideker,Hannah Carter,Elizabeth A Winzeler

Placenta 85:32-39 PubMed31445347

2019

The immunolocalization of Galectin-1 and Progesterone-Induced Blocking Factor (PIBF) in equine trophoblast: Possible roles in trophoblast invasion and the immunological protection of pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Wilsher,J R Newcombe,W R Twink Allen

View all publications

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Anti-PIBF抗体

Anti-PIBF antibody

Immunoprecipitation - Anti-PIBF antibody (AB72118)

Western blot - Anti-PIBF antibody (AB72118)