Anti-PI4 kinase beta/PI4KB抗体(ab134756)
Key features and details
- Rabbit polyclonal to PI4 kinase beta/PI4KB
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PI4 kinase beta/PI4KB抗体
参阅全部 PI4 kinase beta/PI4KB 一抗 -
描述
兔多克隆抗体to PI4 kinase beta/PI4KB -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Baboon, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7.0 to 8.0 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134756于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 91 kDa.
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IP |
Use 2-10 µg for 1000 µg of chromatin.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 91 kDa. |
IP
Use 2-10 µg for 1000 µg of chromatin. |
靶标
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功能
Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking. -
组织特异性
Widely expressed with highest levels in heart, skeletal muscle, pancreas, testis and ovary. Weakly expressed in liver. -
序列相似性
Belongs to the PI3/PI4-kinase family. Type III PI4K subfamily.
Contains 1 PI3K/PI4K domain.
Contains 1 PIK helical domain. -
细胞定位
Endomembrane system. Mitochondrion outer membrane. Rough endoplasmic reticulum membrane. Golgi apparatus. Cytoplasm, perinuclear region. Found in the outer membrane of mitochondria and membranes of the rough endoplasmic reticulum. Recruited to the Golgi complex by the small GTPase ARF to stimulate the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) on the Golgi complex. - Information by UniProt
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数据库链接
- Entrez Gene: 5298 Human
- Omim: 602758 Human
- SwissProt: Q5VWB9 Human
- SwissProt: Q5VWC0 Human
- SwissProt: Q5VWC1 Human
- SwissProt: Q9UBF8 Human
- Unigene: 632465 Human
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别名
- DKFZp686O1820 antibody
- FLJ30129 antibody
- NPIK antibody
see all
图片
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All lanes : Anti-PI4 kinase beta/PI4KB antibody (ab134756) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 10 seconds
Western Blot Gel 4-8% -
Lane 1: Detection of Human PI4 kinase beta/PI4KB by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab134756 at 6 µg/mg lysate for IP.
Lane 2: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized a downstream epitope of PI4 kinase beta/PI4KB.
Lane 3: Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab134756 at 1 µg/ml.
Western Blot Gel 4-8%
Developed using the ECL technique with an exposure time of 3 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab134756 被引用在 1 文献中.
- Li Z et al. PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3. Cell Rep 29:2229-2242.e4 (2019). PubMed: 31747597