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AB109418

重组Anti-PI4 kinase beta/PI4KB抗体[EPR3758(2)]

Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)]

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(3 Publications)

Rabbit Recombinant Monoclonal PI4 kinase beta/PI4KB antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

查看别名

PIK4CB, PI4KB, Phosphatidylinositol 4-kinase beta, PI4K-beta, PI4Kbeta, PtdIns 4-kinase beta, NPIK, PI4K92, PI4KIII

1 Images
Western blot - Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] (AB109418)
  • WB

Unknown

Western blot - Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] (AB109418)

All lanes:

Western blot - Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] (ab109418) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Predicted band size: 91 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-PI4 kinase beta/PI4KB antibody [EPR3758(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3758(2)

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

PI4 kinase beta also known as PI4KB is an important enzyme involved in lipid signaling. It catalyzes the phosphorylation of phosphatidylinositol (PI) to produce phosphatidylinositol 4-phosphate (PI4P) an essential lipid for various cellular processes. PI4KB has a molecular mass of approximately 92 kDa. This enzyme shows expression in various tissues including the brain liver and pancreas. In cells PI4KB predominantly localizes at the Golgi apparatus and some plasma membrane sites.
Biological function summary

The enzyme plays a role in maintaining proper intracellular membrane structure and function. It operates as part of a larger complex involving additional elements such as the small GTPase ARF1 and the scaffold protein ACBD3 which are necessary for its recruitment and activity at Golgi membranes. PI4KB facilitates vesicular trafficking and supplies substrates for the production of other phosphoinositides impacting diverse cellular events like cell growth and signal transduction.

Pathways

PI4KB is an important participant in the phosphoinositide signaling pathway. This pathway regulates membrane dynamics and trafficking influencing cell signaling and membrane identity. PI4KB interacts with proteins such as PI4K2A and PI4K2B in the synthesis of different phosphoinositides which are components of the signaling cascade that include PI3K/AKT pathway having implications in cell growth and survival.

PI4KB has associations with certain infectious diseases and neurological disorders. The enzyme is linked to hepatitis C virus (HCV) replication where it interacts with viral proteins like NS5A facilitating the formation of replication complexes. Furthermore PI4KB has links to neurodegenerative disorders like Alzheimer's disease possibly through its influence on vesicular trafficking and lipid metabolism. These associations highlight its importance in disease mechanisms and as a potential therapeutic target.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Phosphorylates phosphatidylinositol (PI) in the first committed step in the production of the second messenger inositol-1,4,5,-trisphosphate (PIP). May regulate Golgi disintegration/reorganization during mitosis, possibly via its phosphorylation. Involved in Golgi-to-plasma membrane trafficking (By similarity) (PubMed : 10559940, PubMed : 11277933, PubMed : 12749687, PubMed : 9405935). May play an important role in the inner ear development.. (Microbial infection) Plays an essential role in Aichi virus RNA replication (PubMed : 22124328, PubMed : 22258260, PubMed : 27989622). Recruited by ACBD3 at the viral replication sites (PubMed : 22124328, PubMed : 27989622).. (Microbial infection) Required for cellular spike-mediated entry of human coronavirus SARS-CoV.
See full target information PI4KB
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 40:e105990 PubMed34019311

2021

NPC1 regulates the distribution of phosphatidylinositol 4-kinases at Golgi and lysosomal membranes.

Applications

Unspecified application

Species

Unspecified reactive species

Candice Kutchukian,Oscar Vivas,Maria Casas,Julia G Jones,Scott A Tiscione,Sergi Simó,Daniel S Ory,Rose E Dixon,Eamonn J Dickson

Molecular medicine reports 12:6738-44 PubMed26328522

2015

STAT3‑regulated long non‑coding RNAs lnc‑7SK and lnc‑IGF2‑AS promote hepatitis C virus replication.

Applications

WB

Species

Unspecified reactive species

Yulin Xiong,Ming Jia,Jing Yuan,Changjiang Zhang,Yan Zhu,Xuemei Kuang,Lin Lan,Xiaohong Wang

Journal of virology 87:11031-46 PubMed23926333

2013

The Golgi protein ACBD3, an interactor for poliovirus protein 3A, modulates poliovirus replication.

Applications

Unspecified application

Species

Unspecified reactive species

François Téoulé,Cynthia Brisac,Isabelle Pelletier,Pierre-Olivier Vidalain,Sophie Jégouic,Carmen Mirabelli,Maël Bessaud,Nicolas Combelas,Arnaud Autret,Frédéric Tangy,Francis Delpeyroux,Bruno Blondel
View all publications
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