重组Anti-PI 3 Kinase p85 beta抗体[EPR18416] (ab180967)
![Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-409563-anti-pi-3-kinase-p85-beta-antibody-epr18416-western-blot-hek-293t-lysates.jpg "Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18416] to PI 3 Kinase p85 beta
- Suitable for: IHC-P, WB, ICC/IF, IP
- Knockout validated
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-PI 3 Kinase p85 beta抗体[EPR18416]
参阅全部 PI 3 Kinase p85 beta 一抗 -
描述
兔单克隆抗体[EPR18416] to PI 3 Kinase p85 beta -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IF, IPmore details
不适用于: Flow Cyt -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HAP1, HeLa, Jurkat, HEK293 cell lysates; Human fetal brain and fetal kidney lysates; Rat brain and spleen lysates; PC12 cell lysate. IHC-P: Human colonic adenocarcinoma, Human colon, rat testis tissues. ICC/IF: HeLa and Jurkat cells. IP: Jurkat cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18416 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180967于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| WB |
1/2000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
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| ICC/IF |
1/500.
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| IP |
1/80.
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| 说明 |
|---|
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IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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WB
1/2000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). |
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ICC/IF
1/500. |
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IP
1/80. |
靶标
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功能
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. -
疾病相关
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 -
序列相似性
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH2 2 domain is required for interaction with FBXL2 and PTPN13. -
翻译后修饰
Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
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数据库链接
- Entrez Gene: 5296 Human
- Entrez Gene: 29741 Rat
- Omim: 603157 Human
- SwissProt: O00459 Human
- SwissProt: Q63788 Rat
- Unigene: 371344 Human
- Unigene: 22497 Rat
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别名
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
图片
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Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)All lanes : Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967) at 1/2000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PIK3R2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 82 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Lanes 1- 2: Merged signal (red and green). Green - ab180967 observed at 85 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab180967 was shown to react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type HEK-293T and PIK3R2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab180967 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246102-anti-pi-3-kinase-p85-beta-antibody-epr18416-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PI 3 Kinase p85 beta using ab180967 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Cytoplasm staining on epithelial cells of Human colon was observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246099-anti-pi-3-kinase-p85-beta-antibody-epr18416-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling PI 3 Kinase p85 beta with ab180967 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and cytoplasmic staining on HeLa cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab180967 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-276546-anti-pi-3-kinase-p85-beta-antibody-epr18416-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PIK3R2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab180967 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.ab180967 was shown to specifically react with PIK3R2 (KO) when PIK3R2 (KO) knockout samples were used. Wild-type and PIK3R2 (KO) knockout samples were subjected to SDS-PAGE. Ab180967 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 2000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246105-anti-pi-3-kinase-p85-beta-antibody-epr18416-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)All lanes : Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967) at 1/2000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
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![Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246104-anti-pi-3-kinase-p85-beta-antibody-epr18416-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)All lanes : Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
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![Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246103-anti-pi-3-kinase-p85-beta-antibody-epr18416-western-blot.jpg)
Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)All lanes : Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967) at 1/2000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat spleen lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
Exposure time: 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246101-anti-pi-3-kinase-p85-beta-antibody-epr18416-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling PI 3 Kinase p85 beta using ab180967 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Nucleus and cytoplasm staining on tumor cells of colonic adenocarcinoma was observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246100-anti-pi-3-kinase-p85-beta-antibody-epr18416-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling PI 3 Kinase p85 beta using ab180967 at 1/100 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Nucleus and cytoplasm staining on spermatogenic cell of rat testis was observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246098-anti-pi-3-kinase-p85-beta-antibody-epr18416-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PI 3 Kinase p85 beta with ab180967 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and weakly cytoplasmic staining on Jurkat cell line.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:
1. ab180967 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution. -
![Immunoprecipitation - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-246096-anti-pi-3-kinase-p85-beta-antibody-epr18416-immunoprecipitation.jpg)
Immunoprecipitation - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)PI 3 Kinase p85 beta was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab180967 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab180967 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell lysate10 µg (Input).
Lane 2: ab180967 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab180967 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 10 seconds.
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![Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)](https://www.abcam.cn/ps/products/180/ab180967/Images/ab180967-13-benefits-of-recombinant-antibodies.png)
Anti-PI 3 Kinase p85 beta antibody [EPR18416] (ab180967)
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (29)
ab180967 被引用在 29 文献中.
- Hu Q et al. The Antimicrobial Peptide Esculentin-1a(1-21)NH2 Stimulates Wound Healing by Promoting Angiogenesis through the PI3K/AKT Pathway. Biol Pharm Bull 46:382-393 (2023). PubMed: 36385013
- Jiang Z et al. Detection of CSTF2 by nano fluorescent probe and its correlation with malignant biological characteristics in liver cancer. Am J Cancer Res 13:5577-5589 (2023). PubMed: 38058835
- Zhang D et al. Upregulation of miR-144-3p alleviates Doxorubicin-induced heart failure and cardiomyocytes apoptosis via SOCS2/PI3K/AKT axis. Chem Biol Drug Des 101:24-39 (2023). PubMed: 35730258
- Zhang Y et al. MicroRNA-126 and VEGF enhance the function of endothelial progenitor cells in acute myocardial infarction. Exp Ther Med 23:142 (2022). PubMed: 35069823
- Sibilano M et al. Platelet-Derived miR-126-3p Directly Targets AKT2 and Exerts Anti-Tumor Effects in Breast Cancer Cells: Further Insights in Platelet-Cancer Interplay. Int J Mol Sci 23:N/A (2022). PubMed: 35628294