Anti-PI 3 Kinase p85 beta抗体(ab131067)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p85 beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-PI 3 Kinase p85 beta抗体
参阅全部 PI 3 Kinase p85 beta 一抗 -
描述
兔多克隆抗体to PI 3 Kinase p85 beta -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human PI 3 Kinase p85 beta aa 447-461 (internal sequence).
Sequence:KVYHQQYQDKSREYD
-
阳性对照
- WB: Rat testis tissue lysate and HeLa and 293T whole cell lysates. IHC-P: Human mammary cancer tissue.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab131067于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
|
|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
-
功能
Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. -
疾病相关
Megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome 1 -
序列相似性
Belongs to the PI3K p85 subunit family.
Contains 1 Rho-GAP domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH2 2 domain is required for interaction with FBXL2 and PTPN13. -
翻译后修饰
Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).
Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2. - Information by UniProt
-
数据库链接
- Entrez Gene: 5296 Human
- Entrez Gene: 18709 Mouse
- Entrez Gene: 29741 Rat
- Omim: 603157 Human
- SwissProt: O00459 Human
- SwissProt: O08908 Mouse
- SwissProt: Q63788 Rat
- Unigene: 371344 Human
see all -
别名
- p85 antibody
- p85 beta antibody
- P85B antibody
see all
图片
-
Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml
Lane 1 : Rat testis tissue lysate at 50 µg
Lane 2 : 293T whole cell lysate at 40 µg
Lane 3 : HeLa whole cell lysate at 40 µg
Predicted band size: 81 kDa
Observed band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody (ab131067)Immunohistochemical analysis of paraffin embedded Human Mammary Cancer Tissue labelled with ab131067 PI 3 Kinase p85 beta antibody at 1 µg/ml. -
Western blot - Anti-PI 3 Kinase p85 beta antibody (ab131067)All lanes : Anti-PI 3 Kinase p85 beta antibody (ab131067) at 0.5 µg/ml
Lane 1 : mouse liver tissue lysates
Lane 2 : mouse brain tissue lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : goat anti-rabbit IgG-HRP at 1/10000 dilution
Predicted band size: 81 kDa
数据表及文件
-
SDS download
-
Datasheet download
文献 (6)
ab131067 被引用在 6 文献中.
- Sun R et al. Pink Lotus Essential Oil and Alleviates on Free Fatty Acid Induced Steatosis in HepG2 Cells via PI3K/Akt and NF-κB Pathways. J Oleo Sci 71:95-104 (2022). PubMed: 35013040
- Zhang L et al. Exosomes from microRNA-126 overexpressing mesenchymal stem cells promote angiogenesis by targeting the PIK3R2-mediated PI3K/Akt signalling pathway. J Cell Mol Med 25:2148-2162 (2021). PubMed: 33350092
- Yuan H et al. Circ_0000376 downregulation inhibits the progression of non-small cell lung cancer by mediating the miR-488-3p/BRD4 axis and the PI3K/PKB signaling pathway. Histol Histopathol 36:1309-1324 (2021). PubMed: 34730838
- Jia Z et al. miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells. Oncol Lett 15:7369-7375 (2018). PubMed: 29725450
- Song L et al. MicroRNA-126 inhibits proliferation and metastasis by targeting pik3r2 in prostate cancer. Mol Med Rep 13:1204-10 (2016). PubMed: 26677064
- Cao WJ et al. Therapeutic Angiogenesis by Ultrasound-Mediated MicroRNA-126-3p Delivery. Arterioscler Thromb Vasc Biol 35:2401-11 (2015). PubMed: 26381870