JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB278545

重组Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199)抗体[PI3KY458-1A11]

Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

5

(1 Review)

|

(152 Publications)

Anti-PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545) is a rabbit monoclonal antibody detecting PI 3 Kinase p85 alpha in Western Blot, Flow Cytometry. Suitable for Human, Mouse.

- Recombinant format for unrivalled batch-batch consistency
- Over 50 publications

查看别名

GRB1, PIK3R1, Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-alpha, PtdIns-3-kinase regulatory subunit p85-alpha

4 Images
Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells secondary antibody only negative control (blue) or untreated (red) or treated with pervanadate (green) using ab278545 at 0.1 µg/ml.

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Peptide blocking flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only negative control (light blue) or treated with imatinib (red) or with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278545 at 0.1 μg/ml.

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cells secondary antibody only negative control (blue) or 0.1 µg/mL of isotype control (orange) or treated with imatinib (red) or with pervanadate (green) using ab278545 at 0.1 µg/ml.

Western blot - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)
  • WB

Supplier Data

Western blot - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (AB278545)

Western blot analysis of NIH3T3 cell extract, untreated or treated with H2O2 with anti-Phospho-PI3Kinase p85 (Tyr458)/p55 (Tyr199) antibody ab278545. Loading control : AKT (Protein kinase B (PKB)).

All lanes:

Western blot - Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545) at 0.5 µg/mL

Lane 1:

Untreated NIH/3T3 (mouse embryo fibroblast cell line) cell extract

Lane 2:

H2O2-treated NIH/3T3 (mouse embryo fibroblast cell line) cell extract

Observed band size: 85 kDa,70 kDa

false

不同偶联物与剂型 (3)

  • 660 APC

    APC Anti-PI3K p85 (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11]

  • 519 FITC

    FITC Anti-PI 3 Kinase p85 alpha (pY467) + PI3 Kinase p55 (pY199) antibody [PI3KY458-1A11]

  • 578 PE

    PE Anti-PI 3 Kinase p85 alpha (pY467) + PI3 Kinase p55 (pY199) antibody [PI3KY458-1A11]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

PI3KY458-1A11

亚型

IgG

轻链类型

kappa

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB, Flow Cyt

applications

免疫原

Synthetic Peptide within Human PIK3R1 phospho Y467. The exact immunogen used to generate this antibody is proprietary information.

P27986

特异性

Recent data suggests this antibody can recognize PI 3 Kinase p85 and p55.

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1 µg/mL", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }
style
left-column

产品详情

What is this antibody validated in?
Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) antibody [PI3KY458-1A11] (ab278545) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt) in Human, Mouse samples.

What is the molecular weight of PI 3 Kinase p85 alpha?
Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) [PI3KY458-1A11] (ab278545) specifically detects a band for PI 3 Kinase p85 alpha (UniProt: Q92569) at a molecular weight of 84kDa.

Trusted by the scientific community
Anti- PI 3 Kinase p85 alpha (phospho Y467) + PI3 Kinase p55 (phospho Y199) [PI3KY458-1A11] (ab278545) was first used in a scientific publication in 2021 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [PI3KY458-1A11] also available for your convenience: ab278545, PE - ab278691, FITC - ab278692, APC - ab278733

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
存储溶液
Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The PI 3 Kinase p85 alpha also known as PI3K p85 or PI3-kinase p85 is a regulatory subunit part of the phosphoinositide 3-kinases (PI3Ks) complex which is integral to cellular functions. The p85 alpha form partners with the catalytic subunit to form PI3K p55 known for its significant role in signaling. This subunit exhibits a molecular weight of approximately 85 kDa. It shows expression in various tissues including liver muscle and brain where it aids in cellular communication responding to extracellular signals.
Biological function summary

PI 3 Kinase p85 alpha plays an essential role in intracellular signaling where it functions as part of the PI3K complex. It functions to regulate metabolic processes by interacting with phosphatidylinositol lipids in the cell membrane. PI3K p55 form contributes to cell growth survival and proliferation often collaborating with the catalytic subunit p110 to execute these functions. The phospho PI3K form becomes active upon receiving external growth factors facilitating downstream signaling.

Pathways

The involvement of PI 3 Kinase p85 alpha extends to critical cellular pathways like the PI3K/AKT pathway and the insulin signaling pathway. It engages with proteins such as AKT (protein kinase B) and IRS-1 linking to various cellular functions including metabolism protein synthesis and cellular proliferation. The activity of PI3K p55 form regulates these pathways by influencing enzyme activity and pathways associated with cell survival and apoptosis.

Dysregulation of PI 3 Kinase p85 alpha associates closely with cancers and metabolic disorders like type 2 diabetes. Aberrations in the p55 form link to tumorigenesis due to its role in uncontrolled cell proliferation. PI3K pathway disorders also relate to insulin resistance where the malfunction of PI3K components disrupts normal glucose uptake and insulin signaling. This target when interacting with proteins like PTEN further influences the development of cancer highlighting its critical role in both oncogenic and metabolic pathologies.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed : 17626883, PubMed : 19805105, PubMed : 7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed : 20348923).
See full target information PIK3R1 phospho Y467
style
left-column

文献 (152)

Recent publications for all applications. Explore the full list and refine your search

Physiological reports 13:e70553 PubMed40969143

2025

Midkine promotes PDGF-BB-induced proliferation, migration, and glycolysis of airway smooth muscle cells via the PI3K Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Xu,Wenli Li,Jun Shi,Yanfang Guo

Journal of clinical and translational hepatology 13:555-567 PubMed40937080

2025

CCNE1 Promotes the Progression of Hepatic Precancerous Lesion and the Malignant Phenotype of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xue Hu,Lichao Yao,Wenzhi Guo

Journal of diabetes research 2025:9601959 PubMed40933728

2025

Myristoylated Cathelicidin-DM Fused With ANG1-7: A Novel Self-Assembling Antimicrobial Peptide for the Treatment and Mechanism of Diabetic Infected Wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Rongqin Feng,Peng Wang,Li Fan,He Lu,Danna Yao,Panpan Sun,Zhonghua Liu,Fu Han,Xiaozhi Bai,Xuekang Yang,Juntao Han

European journal of histochemistry : EJH 69: PubMed40832995

2025

Aloe-emodin ameliorates chronic kidney disease fibrosis by inhibiting PI3K-mediated signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Chen,Wenhui Zhu,Yao Chen,Jingying Shang,Wenfan Wang,Xiaoming Yan,Peng Liu,Yabin Zhou

Scientific reports 15:29741 PubMed40804251

2025

HSF1 in macrophages suppressed the progression of asthma via modulating SIRPα/SHP2-Dectin-1/ SYK mediated ROS and inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Yingqian Zhang,Huifang Wu,Jingli Zhang,Yingxue Wang,Huifeng Zhang

Journal of orthopaedic surgery and research 20:745 PubMed40775331

2025

Yam polysaccharide promoted bone differentiation of MC3T3-E1 cells through targeted Inhibition of CASP3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Qiting Yu,LinHui Liu,TaiPing Jiang,Zhiyu Guan,ZhaoMing Liu

Bioactive materials 53:459-479 PubMed40747455

2025

Metformin carbon dots-based osteogenic and protein delivery system to promote bone regeneration in periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wei,Kai Wang,Yongkai Li,Jiao Huang,Ping Deng,Xianbo Xia,Cong Yang,Ling Xu,Junji Xu

Scientific reports 15:27739 PubMed40730854

2025

CIAPIN1 promotes survival, proliferation, migration and glycolysis of endometrial cancer cells through PI3K/Akt pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huanmei Sun,Juan Shao,Yingchun Duan,Jiashi Gu

Iranian journal of basic medical sciences 28:946-954 PubMed40703755

2025

Schisandrin B regulates the SIRT1/PI3K/Akt signaling pathway to ameliorate Ang II-infused cardiac fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Zhang,Mengqing Shi,Zhongying Xing,Jie Su,Yuying Gu,Zhongping Ning

Chemical biology & drug design 105:e70135 PubMed40504801

2025

Anti-Osteoporosis Activity of Lycopene Through ESR1: Network Pharmacology, Molecular Docking, Imaging Technology, and Experimental Validation.

Applications

Unspecified application

Species

Unspecified reactive species

Benqian Zhao,Lulu Chen,Wei Wang,Weinan Xu,Bing Xu
View all publications
style
left-column
style
left-column
style
right-column

搭配使用产品

Assay Kits

AB318932

Human PI 3 Kinase p85 alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human PI 3 Kinase p85 alpha ELISA Kit (AB318932)

  • 0
  • 0
Primary Antibodies

AB38449

Anti-AKT (phospho T308) antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKT (phospho T308) antibody (AB38449)

  • 3
  • 5
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB191606

Anti-PI 3 Kinase p85 alpha antibody [EPR18702]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [EPR18702] (AB191606)

  • 5
  • 2

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com