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重组RabMAb

重组Anti-PI 3 Kinase p85 alpha抗体[SP62] (ab225720)

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Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
  • Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP62] to PI 3 Kinase p85 alpha
  • Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-PI 3 Kinase p85 alpha抗体[SP62]
    参阅全部 PI 3 Kinase p85 alpha 一抗

  • 描述

    兔单克隆抗体[SP62] to PI 3 Kinase p85 alpha

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Cow

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human placenta, lung adenocarcinoma, pancreatic adenocarcinoma, bladder transitional cell carcinoma, colon adenocarcinoma, melanoma, prostate carcinoma, stomach adenocarcinoma, testis and breast adenocarcinoma tissues. Flow Cyt (Intra): NIH/3T3, PC-12 and MCF7 cells. ICC/IF: NIH/3T3 and MCF7 cells.

  • 常规说明

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab225720于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

Primary incubation for 30 minutes at 4°C.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

Primary incubation for 30 minutes at 4°C.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 30 minutes at room temperature.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.

  • 组织特异性

    Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).

  • 序列相似性

    Belongs to the PI3K p85 subunit family.
    Contains 1 Rho-GAP domain.
    Contains 2 SH2 domains.
    Contains 1 SH3 domain.

  • 结构域

    The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.

  • 翻译后修饰

    Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
    Phosphorylated. Dephosphorylated by PTPRJ.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • GRB1 antibody
    • p85 alpha antibody
    • p85 antibody
    • P85A_HUMAN antibody
    • Phosphatidylinositol 3 kinase associated p 85 alpha antibody
    • Phosphatidylinositol 3 kinase regulatory 1 antibody
    • Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 1 (p85 alpha) antibody
    • Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha antibody
    • Phosphatidylinositol 3-kinase regulatory subunit alpha antibody
    • Phosphoinositide 3 kinase, regulatory subunit 1 (alpha) antibody
    • PI3 kinase p85 antibody
    • PI3 kinase p85 subunit alpha antibody
    • PI3-kinase regulatory subunit alpha antibody
    • PI3-kinase subunit p85-alpha antibody
    • PI3K antibody
    • PI3K p85 antibody
    • PI3K regulatory subunit alpha antibody
    • Pik3r1 antibody
    • PtdIns 3 kinase p85 alpha antibody
    • PtdIns-3-kinase regulatory subunit alpha antibody
    • PtdIns-3-kinase regulatory subunit p85-alpha antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling PI 3 Kinase p85 alpha with purified ab225720. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Flow cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) labeling PI 3 Kinase p85 alpha with purified ab225720 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 (10 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human placenta tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Flow cytometry analysis of NIH/3T3 (mouse embryo) labeling PI 3 Kinase p85 alpha with purified ab225720 at 1/250 dilution (10.32 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Flow cytometry analysis of MCF-7 (human breast carcinoma) labeling PI 3 Kinase p85 alpha with purified ab225720 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabelled cells (blue).

  • Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Flow Cytometry (Intracellular) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cell line labeling PI 3 Kinase p85 alpha with ab225720 (green) compared with a negative control of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human testis tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

    Formalin-fixed, paraffin-embedded human breast adenocarcinoma tissue stained for PI 3 Kinase p85 alpha using ab225720 at 1/100 dilution in immunohistochemical analysis.

  • Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)
    Anti-PI 3 Kinase p85 alpha antibody [SP62] (ab225720)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab225720？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab225720 被引用在 2 文献中.

  • Hecht JT  et al. Resveratrol Reduces COMPopathy in Mice Through Activation of Autophagy. JBMR Plus 5:e10456 (2021). PubMed: 33778324
  • Duman BB  et al. Evaluation of PTEN, PI3K, MTOR, and KRAS expression and their clinical and prognostic relevance to differentiated thyroid carcinoma. Contemp Oncol (Pozn) 18:234-40 (2014). PubMed: 25258580

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