Anti-PI 3 Kinase p85 alpha + Gamma抗体(ab74136)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p85 alpha + Gamma
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-PI 3 Kinase p85 alpha + Gamma抗体 -
描述
兔多克隆抗体to PI 3 Kinase p85 alpha + Gamma -
宿主
Rabbit -
特异性
PI3 kinase p85 alpha and gamma. -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human, African green monkey -
免疫原
Synthetic non-phosphopeptide derived from human PI 3 Kinase p85 alpha around the phosphorylation site of tyrosine 468 (R-L-YP-E-E).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74136于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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| IHC-P |
1/50 - 1/100.
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| ICC/IF | (1) |
1/500.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
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IHC-P
1/50 - 1/100. |
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ICC/IF
1/500. |
靶标
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相关性
The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that generates phosphatidylinositol 3, 4, 5-triphosphate in response to receptor activation in many signal transduction pathways. Class IA PI3Ks exist as a heterodimer of a catalytic 110 kDa (p110) and a regulatory p85 subunit (e.g. p85 alpha). p85 alpha is an adaptor molecule that regulates the activity of the catalytic p110 subunit by binding to phosphorylated receptor tyrosine kinases (RTKs) through its SH2 domain and mediating the interaction between p110 and the plasma membrane. p85 alpha is necessary for insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 5295 Human
- Entrez Gene: 8503 Human
- Omim: 171833 Human
- Omim: 606076 Human
- SwissProt: P27986 Human
- SwissProt: Q92569 Human
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别名
- GRB1 antibody
- p55 antibody
- p55 GAMMA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab74136, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)Immunofluorescence analysis of HeLa cells using ab74136, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide. -
Western blot - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)All lanes : Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136) at 1/500 dilution
Lane 1 : Extracts from COS7 cells, treated
with H2O2 (100µM, 30mins)
Lane 2 : Extracts from COS7 cells, treated
with H2O2 (100µM, 30mins) with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 70 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p85 alpha + Gamma antibody (ab74136)ab74136 at 1/200 dilution staining PI 3 Kinase p85 alpha + Gamma in mouse embryonic stem cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized in 1:1 methanol, acetone and blocked in 1% serum for 30 minutes at 25°C. The primary antibody was used at 1/100 dilution in Goat block dillution buffer and incubated with sample at 25°C for 1 hours. An Alexa Fluor® 488 conjugated Goat polyclonal to rabbit IgG (H+L) was used as secondary at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab74136 被引用在 12 文献中.
- Liu W et al. Dynamic characterization of intestinal metaplasia in the gastric corpus mucosa of Atp4a-deficient mice. Biosci Rep 40:N/A (2020). PubMed: 31904088
- Liu Z et al. Shenzhiling Oral Liquid Protects STZ-Injured Oligodendrocyte through PI3K/Akt-mTOR Pathway. Evid Based Complement Alternat Med 2020:4527283 (2020). PubMed: 32774416
- Wu S et al. In Vitro Evaluation of the Neuroprotective Effect of Panax notoginseng Saponins by Activating the EGFR/PI3K/AKT Pathway. Evid Based Complement Alternat Med 2020:1403572 (2020). PubMed: 32802113
- Dou F et al. Aloe-Emodin Ameliorates Renal Fibrosis Via Inhibiting PI3K/Akt/mTOR Signaling Pathway In Vivo and In Vitro. Rejuvenation Res 22:218-229 (2019). PubMed: 30215298
- Xie L et al. Inhibiting the PI3K/AKT/NF-?B signal pathway with nobiletin for attenuating the development of osteoarthritis: in vitro and in vivo studies. Food Funct 10:2161-2175 (2019). PubMed: 30938722