重组Anti-PI 3 Kinase p110 delta抗体[Y387] (ab32401)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y387] to PI 3 Kinase p110 delta
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-PI 3 Kinase p110 delta抗体[Y387]
参阅全部 PI 3 Kinase p110 delta 一抗 -
描述
兔单克隆抗体[Y387] to PI 3 Kinase p110 delta -
宿主
Rabbit -
特异性
ab32401 recognises PI3-Kinases (PI3-Ks). -
经测试应用
适用于: WB, ICC/IF, IPmore details
不适用于: Flow Cyt or IHC -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human PI 3 Kinase p110 delta aa 250-350. The exact sequence is proprietary.
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阳性对照
- Jurkat whole cell lysate (ab7899) and HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
Y387 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab32401于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 119 kDa).
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ICC/IF |
1/250 - 1/500.
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IP |
1/50.
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说明 |
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WB
1/500. Detects a band of approximately 110 kDa (predicted molecular weight: 119 kDa). |
ICC/IF
1/250 - 1/500. |
IP
1/50. |
靶标
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组织特异性
Expressed predominantly in leukocytes. -
通路
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
序列相似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
翻译后修饰
Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5293 Human
- Omim: 602839 Human
- SwissProt: O00329 Human
- Unigene: 518451 Human
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别名
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
- 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
- APDS antibody
see all
图片
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Anti-PI 3 Kinase p110 delta antibody [Y387] (ab32401) at 1/500 dilution + Jurkat cell lysate
Predicted band size: 119 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Immunofluorescent staining of HeLa cells using ab32401 at 1/250 dilution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab32401 被引用在 13 文献中.
- Agnetti J et al. PI3Kδ activity controls plasticity and discriminates between EMT and stemness based on distinct TGFβ signaling. Commun Biol 5:740 (2022). PubMed: 35879421
- Compagno M et al. Phosphatidylinositol 3-kinase d blockade increases genomic instability in B cells. Nature 542:489-493 (2017). PubMed: 28199309
- Milara J et al. Non-neuronal cholinergic system contributes to corticosteroid resistance in chronic obstructive pulmonary disease patients. Respir Res 17:145 (2016). IP ; Human . PubMed: 27825347
- Mendizabal-Zubiaga J et al. Cannabinoid CB1 Receptors Are Localized in Striated Muscle Mitochondria and Regulate Mitochondrial Respiration. Front Physiol 7:476 (2016). IP ; Mouse . PubMed: 27826249
- Kitatani K et al. Ceramide limits phosphatidylinositol-3-kinase C2ß-controlled cell motility in ovarian cancer: potential of ceramide as a metastasis-suppressor lipid. Oncogene 35:2801-12 (2016). PubMed: 26364609