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AB109006

重组Anti-PI 3 Kinase p110 delta抗体[EPR386]

Anti-PI 3 Kinase p110 delta antibody [EPR386]

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(22 Publications)

Rabbit Recombinant Monoclonal PI 3 Kinase p110 delta antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 22 publications.

查看别名

PI3-kinase subunit delta, PI3K-delta, PI3Kdelta, PtdIns-3-kinase subunit delta, PtdIns-3-kinase subunit p110-delta, p110delta, PIK3CD

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)

Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PI 3 Kinase p110 delta with Purified ab109006 at 1 : 150 dilution (9.8 μg/ml). Cells were fixed in 100% Methanol and permeabilized with none. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)
  • WB

Unknown

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1.5 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg

Lane 2:

RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg

Lane 3:

Rat spleen lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 119 kDa

false

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)
  • WB

Lab

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)

Lane 1 : Wild type HAP1 whole cell lysate (40 μg)
Lane 2 : PIK3CD knockout HAP1 whole cell lysate (40 μg)
Lane 3 : Jurkat whole cell lysate (40 μg)
Lane 4 : K562 whole cell lysate (40 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab109006 observed at 120 kDa. Red - loading control, ab8245, observed at 37 kDa.

Unpurified ab109006 was shown to specifically react with PIK3CD in wild-type HAP1 cells. No band was observed when PIK3CD knockout samples were used. Wild-type and PIK3CD knockout samples were subjected to SDS-PAGE. ab109006 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

All lanes:

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006)

Predicted band size: 119 kDa

Observed band size: 120 kDa

false

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)
  • WB

Unknown

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (AB109006)

All lanes:

Western blot - Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

RAW 264.7 cell lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat kidney tissue lysate at 10 µg

Lane 7:

Rat spleen tissue lysate at 10 µg

Predicted band size: 119 kDa

false

不同偶联物与剂型 (1)

  • Carrier free

    Anti-PI 3 Kinase p110 delta antibody [EPR386] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR386

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" } } }
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产品详情

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Phosphoinositide 3-kinase p110 delta also known as PI3Kδ or PIK3CD is a catalytic subunit of the class I PI3K enzyme complex. It plays a significant role in the phosphorylation of the 3'-hydroxyl group of inositol lipids which acts as a signal transducer. The molecular weight of p110 delta is approximately 110 kDa. This protein is highly expressed in leukocytes especially in B and T lymphocytes. Expression can also be seen in other cell types like neutrophils.
Biological function summary

P110 delta is involved in immune cell signaling as it is part of the class I PI3K complex. PI3Kδ functions in conjunction with p85 regulatory subunits to modulate various intracellular processes. The complex plays an important role in regulating cell growth proliferation and survival by mediating signals from growth factors or cytokines. It is also critical in the development and function of the adaptive immune system.

Pathways

PI3Kδ participates in the PI3K/AKT signaling pathway an important regulatory pathway in cell growth and survival. Another significant pathway involving p110 delta is the B-cell receptor (BCR) signaling pathway which is important for B cell development. Within these pathways PI3Kδ interacts with proteins such as AKT mTOR and BCR-related proteins that propagate downstream signaling events.

Aberrant activity of PI3Kδ has been linked to various immune-related diseases such as chronic lymphocytic leukemia (CLL) and rheumatoid arthritis. The overactivation of PI3Kδ in CLL correlates with enhanced survival and proliferation of malignant B cells. Additionally PI3Kδ dysfunction can influence inflammation pathways contributing to autoimmune conditions. The protein often works in concert with other proteins in these disease contexts such as B cells in CLL where it affects signaling via the B-cell receptor pathway.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed : 9235916). Uses ATP and PtdIns(4,5)P2 (phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) (PubMed : 15135396). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Mediates immune responses. Plays a role in B-cell development, proliferation, migration, and function. Required for B-cell receptor (BCR) signaling. Mediates B-cell proliferation response to anti-IgM, anti-CD40 and IL4 stimulation. Promotes cytokine production in response to TLR4 and TLR9. Required for antibody class switch mediated by TLR9. Involved in the antigen presentation function of B-cells. Involved in B-cell chemotaxis in response to CXCL13 and sphingosine 1-phosphate (S1P). Required for proliferation, signaling and cytokine production of naive, effector and memory T-cells. Required for T-cell receptor (TCR) signaling. Mediates TCR signaling events at the immune synapse. Activation by TCR leads to antigen-dependent memory T-cell migration and retention to antigenic tissues. Together with PIK3CG participates in T-cell development. Contributes to T-helper cell expansion and differentiation. Required for T-cell migration mediated by homing receptors SELL/CD62L, CCR7 and S1PR1 and antigen dependent recruitment of T-cells. Together with PIK3CG is involved in natural killer (NK) cell development and migration towards the sites of inflammation. Participates in NK cell receptor activation. Plays a role in NK cell maturation and cytokine production. Together with PIK3CG is involved in neutrophil chemotaxis and extravasation. Together with PIK3CG participates in neutrophil respiratory burst. Plays important roles in mast-cell development and mast cell mediated allergic response. Involved in stem cell factor (SCF)-mediated proliferation, adhesion and migration. Required for allergen-IgE-induced degranulation and cytokine release. The lipid kinase activity is required for its biological function. Isoform 2 may be involved in stabilizing total RAS levels, resulting in increased ERK phosphorylation and increased PI3K activity.
See full target information PIK3CD
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文献 (22)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical immunology 45:139 PubMed41026257

2025

Homozygous Loss of Function PIK3CD Mutation in Multiple Siblings Leading To B Cell Dysregulation and Autoimmunity.

Applications

Unspecified application

Species

Unspecified reactive species

Huda Alajlan,Amer Al-Mazrou,Hibah Alruwaili,Safia Sumayli,Ali Almehaidib,Khalid Alsaleem,Sawsan Abu Awwad,Hazem Ghebeh,Monther Al-Alwan,Anas M Alazami,Hamoud Al-Mousa

Blood vessels, thrombosis & hemostasis 1:100022 PubMed40765933

2025

Challenges with measuring tissue factor antigen and activity in human plasma.

Applications

Unspecified application

Species

Unspecified reactive species

Nigel Mackman,Ana T A Sachetto

American journal of translational research 17:4689-4700 PubMed40672621

2025

Undemineralized dentin matrix particles accelerate blood vessel formation in a critical-sized skull defect through activating the TGF-β/PI3K signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zu,Xiangwen Zhou,Qingsong Jiang

Cellular and molecular life sciences : CMLS 82:82 PubMed39985671

2025

Class I PI3Ks activate stretch-induced autophagy in trabecular meshwork cells.

Applications

Unspecified application

Species

Unspecified reactive species

Myoung Sup Shim,Ethan J Sim,Kevin Betsch,Vaibhav Desikan,Chien-Chia Su,Diego Pastor-Valverde,Yang Sun,Paloma B Liton

Cell death & disease 15:822 PubMed39537605

2024

SPINK13 acts as a tumor suppressor in hepatocellular carcinoma by inhibiting Akt phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhi Lun,Jie Sun,Ling Wei,Ben Liu,Zhixue Li,Wen Dong,Wenqi Zhao

PLoS pathogens 20:e1012543 PubMed39250507

2024

Trem2/Syk/PI3K axis contributes to the host protection against Toxoplasma gondii-induced adverse pregnancy outcomes via modulating decidual macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Wang,Yining Cao,Songyi Ye,Maoyuan Ding,Wenliang Ge,Yuejin Liang,Jinling Chen

Journal of orthopaedic translation 47:176-190 PubMed39040490

2024

Sargassum polysaccharide attenuates osteoarthritis in rats and is associated with the up-regulation of the ITGβ1-PI3K-AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanzhi Liu,Rui Lin,Haiping Fang,Lixian Li,Min Zhang,Lujiao Lu,Xiang Gao,Jintong Song,Jinsong Wei,Qixian Xiao,Fucheng Zhang,Kefeng Wu,Liao Cui

Cell death & disease 14:188 PubMed36898991

2023

PTPN14 promotes gastric cancer progression by PI3KA/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Bingxin Guan,Sen Liu,Haiting Liu,Lin Song,Guohao Zhang,Ruinan Zhao,Chengjun Zhou,Peng Gao

Medicine 101:e30951 PubMed36254040

2022

Potential pathogenic mechanism of type 1 X-linked lymphoproliferative syndrome caused by a mutation of SH2D1A gene in an infant: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Wang,Yan Wang,Weimin Lu,Lvyan Tao,Yang Xiao,Yuantao Zhou,Xiaoli He,Yu Zhang,Li Li

Cell death discovery 8:197 PubMed35418181

2022

MiR-92a/KLF4/p110δ regulates titanium particles-induced macrophages inflammation and osteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenkang Wen,Sipeng Lin,Changchuan Li,Zhuji Ouyang,Zhong Chen,Shixun Li,Yuxi Huang,Wenqiang Luo,Zhongcan Zheng,Peidong Guo,Manyuan Kuang,Yue Ding
View all publications
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p110 delta antibody (AB200372)

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