Anti-PI 3 Kinase p110 delta抗体(ab1678)
Key features and details
- Rabbit polyclonal to PI 3 Kinase p110 delta
- Suitable for: WB, ICC, IP, ELISA, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-PI 3 Kinase p110 delta抗体
参阅全部 PI 3 Kinase p110 delta 一抗 -
描述
兔多克隆抗体to PI 3 Kinase p110 delta -
宿主
Rabbit -
特异性
Binds to a 110 Kd protein. Not likely to cross react with other isoforms due to sequence dissimilarity. -
经测试应用
适用于: WB, ICC, IP, ELISA, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to PI 3 Kinase p110 delta (C terminal). 20 amino acid C-terminal peptide to Phosphoinositide 3 Kinase p110 delta
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (3) |
Use at an assay dependent concentration.
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ICC |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration. PubMed: 23023391
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|
ELISA |
Use at an assay dependent concentration.
|
|
ICC/IF |
Use a concentration of 1 µg/ml.
|
说明 |
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WB
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. PubMed: 23023391 |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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组织特异性
Expressed predominantly in leukocytes. -
通路
Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. -
序列相似性
Belongs to the PI3/PI4-kinase family.
Contains 1 PI3K/PI4K domain. -
翻译后修饰
Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. - Information by UniProt
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数据库链接
- Entrez Gene: 5293 Human
- Entrez Gene: 18707 Mouse
- Omim: 602839 Human
- SwissProt: O00329 Human
- SwissProt: O35904 Mouse
- Unigene: 518451 Human
- Unigene: 229108 Mouse
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别名
- 5-bisphosphate 3-kinase 110 kDa catalytic subunit delta antibody
- 5-bisphosphate 3-kinase catalytic subunit delta isoform antibody
- APDS antibody
see all
图片
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ICC/IF image of ab1678 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1678, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
数据表及文件
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SDS download
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Datasheet download
文献 (23)
ab1678 被引用在 23 文献中.
- Nieuwenhuis B et al. PI 3-kinase delta enhances axonal PIP3 to support axon regeneration in the adult CNS. EMBO Mol Med 12:e11674 (2020). PubMed: 32558386
- Zhang ZR & Yang N MiR-33a-5p inhibits the growth and metastasis of melanoma cells by targeting SNAI2. Neoplasma 67:813-824 (2020). PubMed: 32305057
- Romeo V et al. Effects of PI3K Inhibition on Afucosylated Antibody-Driven Fc?RIIIa Events and Phospho-S6 Activity in NK Cells. J Immunol 203:137-147 (2019). PubMed: 31092639
- Shin YJ et al. PIP4K2A as a negative regulator of PI3K in PTEN-deficient glioblastoma. J Exp Med 216:1120-1134 (2019). PubMed: 30898893
- Mei XL & Zhong S Long noncoding RNA LINC00520 prevents the progression of cutaneous squamous cell carcinoma through the inactivation of the PI3K/Akt signaling pathway by downregulating EGFR. Chin Med J (Engl) 132:454-465 (2019). PubMed: 30707166