Anti-PI-15抗体(ab133172)
Key features and details
- Goat polyclonal to PI-15
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PI-15抗体 -
描述
山羊多克隆抗体to PI-15 -
宿主
Goat -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Chicken, Cow, Dog, Pig, Zebrafish -
免疫原
Synthetic peptide corresponding to Human PI-15 aa 128-141 (internal sequence) (Cysteine residue).
Sequence:C-RTGRYRSILQLVKP
Database link: NP_056970.1 -
阳性对照
- HeLa cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133172于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa).
1 hour primary incubation is recommended for this product. |
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 29 kDa). 1 hour primary incubation is recommended for this product. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Serine protease inhibitor which displays weak inhibitory activity against trypsin. -
组织特异性
Weakly expressed. Expressed at low level in prostate, mammary gland, salivary gland and thyroid gland. -
序列相似性
Belongs to the CRISP family. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 374241 Chicken
- Entrez Gene: 532863 Cow
- Entrez Gene: 51050 Human
- Entrez Gene: 94227 Mouse
- Entrez Gene: 301489 Rat
- Omim: 607076 Human
- SwissProt: Q98ST6 Chicken
- SwissProt: O43692 Human
see all -
别名
- 25 kDa trypsin inhibitor antibody
- CRISP-8 antibody
- CRISP8 antibody
see all
图片
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Anti-PI-15 antibody (ab133172) at 0.3 µg/ml + HeLa cell lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 29 kDa
Observed band size: 33 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab133172 尚未被引用在任何文献中。