Anti-Phosphotyrosine抗体[IG2] (ab5592)
![ELISA - Anti-Phosphotyrosine antibody [IG2] (ab5592)](https://www.abcam.cn/ps/products/5/ab5592/Images/ab5592-467349-anti-phosphotyrosine-antibody-elisa.jpg "ELISA - Anti-Phosphotyrosine antibody [IG2] (ab5592)")
Key features and details
- Mouse monoclonal [IG2] to Phosphotyrosine
- Suitable for: ELISA, ICC/IF, WB, ICC, Flow Cyt, IP
- Reacts with: Species independent
- Isotype: IgG1
概述
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产品名称
Anti-Phosphotyrosine抗体[IG2]
参阅全部 Phosphotyrosine 一抗 -
描述
小鼠单克隆抗体[IG2] to Phosphotyrosine -
宿主
Mouse -
特异性
ab5592 detects phosphotyrosine from most species and tissues. ab5592 will not react with any other phosphorylated amino acids, proteins or nucleotides. -
经测试应用
适用于: ELISA, ICC/IF, WB, ICC, Flow Cyt, IPmore details -
种属反应性
与反应: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Phosphotyrosine. O-phospho-L-tyrosine and O-phospho-DL-tyramine.
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阳性对照
- HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
单克隆 -
克隆编号
IG2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use a concentration of 1 µg/ml.
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| ICC/IF |
1/50 - 1/200.
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| WB |
1/100 - 1/1000.
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| ICC |
1/50 - 1/200.
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| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| IP |
Use a concentration of 2 µg/ml.
|
| 说明 |
|---|
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ELISA
Use a concentration of 1 µg/ml. |
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ICC/IF
1/50 - 1/200. |
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WB
1/100 - 1/1000. |
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ICC
1/50 - 1/200. |
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Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IP
Use a concentration of 2 µg/ml. |
靶标
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相关性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
图片
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ELISA - Anti-Phosphotyrosine antibody [IG2] (ab5592)
Direct ELISA of a 96 well plate coated with 100 uL per well of phosphotyrosine, phosphoserine or phosphothreonine at 5 µg/mL, incubated with 100 µL per well of ab5592
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![Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)](https://www.abcam.cn/ps/products/5/ab5592/Images/ab5592_1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [IG2] (ab5592)Immunolocalization of phosphotyrosine in HeLa cells using ab5592.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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