重组Anti-Phosphothreonine抗体[EPR22006-23]

• WB

Lab

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (AB218195)

No difference in the signal detected by ab218195 upon pervanadate (a protein tyrosine phosphatase inhibitor) treatment.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1:

Untreated Jurkat (human t cell leukemia t lymphocyte) whole cell lysate at 20 µg

Lane 2:

Jurkat treated with 50 mM pervanadate for 5 minutes, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 6s

• WB

Lab

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (AB218195)

Blocking/Dilution buffer : 5% NFDM/TBST.

Observed MW : Multiple smeared bands.

All lanes:

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1:

Untreated Jurkat (human t cell leukemia T lymphocyte) whole cell lysate (Untreated membrane) at 10 µg

Lane 2:

Jurkat treated with 0.1 μM Calyculin A for 45 minutes, whole cell lysate (Untreated membrane) at 10 µg

Lane 3:

Jurkat treated with 0.1 μM Calyculin A for 45 minutes, whole cell lysate (Phosphatase treated membrane) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (AB218195)

Blocking/Dilution buffer : 5% NFDM/TBST.

Observed MW : Multiple smeared bands.

All lanes:

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 treated with 0.1 uM Calyculin A for 15 minutes, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 59s

• WB

Lab

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (AB218195)

Blocking/Dilution buffer : 5% NFDM/TBST.

Observed MW : Multiple smeared bands.

All lanes:

Western blot - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195) at 1/1000 dilution

Lane 1:

Untreated C6 (rat glial tumor glial cell) whole cell lysate at 10 µg

Lane 2:

C6 treated with 0.1 uM Calyculin A for 30 minutes, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3min

• ELISA

Unknown

ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (AB218195)

ELISA - Anti-Phosphothreonine antibody [EPR22006-23] (ab218195).

Each point in the Y axis represents one peptide of the corresponding protein used in ELISA test. The red asterisk indicates a phosphorylated amino acid site. The X axis is the signal to noise (S/N) ratio calculated by the signal intensity of a phospho peptide and its non-phospho paralog.

Antigens used at 1 μg/ml : TWIST1(pY107); TP73(pY99); SQSTM1 (pS403); NFKB (pS337); Phosphoserine; Phosphothreonine; ADD1(pT445); EIF2AK3(pT982); AURKB(pT232); AKT1(pT308).

Primary antibody ab218195 used at a 1/10,000 dilution.

Secondary antibody Alkaline Phosphatase AffiniPure Goat Anti-Rabbit IgG (H+L) used at a 1/2,500 dilution.