重组Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253)抗体[EP1502Y] (ab81284)
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253) antibody [EP1502Y] (ab81284)](https://www.abcam.cn/ps/products/81/ab81284/Images/ab81284-96471-anti-phospholipase-c-gamma-1-plc-gamma-1-phospho-y1253-antibody-ep1502y-western-blot.jpg "Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253) antibody [EP1502Y] (ab81284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1502Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253)抗体[EP1502Y]
参阅全部 Phospholipase C gamma 1/PLC-gamma-1 一抗 -
描述
兔单克隆抗体[EP1502Y] to Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat + Pervanadate cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1502Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab81284于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/5000. Predicted molecular weight: 149 kDa.
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| 说明 |
|---|
|
WB
1/2000 - 1/5000. Predicted molecular weight: 149 kDa. |
应用说明
Is unsuitable for IHC-P or IP.
靶标
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功能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
序列相似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻译后修饰
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
细胞定位
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 5335 Human
- Omim: 172420 Human
- SwissProt: P19174 Human
- Unigene: 268177 Human
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别名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
图片
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253) antibody [EP1502Y] (ab81284)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253) antibody [EP1502Y] (ab81284) at 1/2000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat + Pervanadate cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 149 kDa
Observed band size: 149 kDa -
![Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253) antibody [EP1502Y] (ab81284)](https://www.abcam.cn/ps/products/81/ab81284/Images/ab81284-2-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase C gamma 1/PLC-gamma-1 (phospho Y1253) antibody [EP1502Y] (ab81284)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab81284 尚未被引用在任何文献中。