重组Anti-Phospholipase C gamma 1/PLC-gamma-1抗体[EPR5358] (ab109501)
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.cn/ps/products/109/ab109501/Images/ab109501-395787-anti-phospholipase-c-gamma-1plc-gamma-1-antibody-epr5358-western-blot-hek-293t-lysates.jpg "Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5358] to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-Phospholipase C gamma 1/PLC-gamma-1抗体[EPR5358]
参阅全部 Phospholipase C gamma 1/PLC-gamma-1 一抗 -
描述
兔单克隆抗体[EPR5358] to Phospholipase C gamma 1/PLC-gamma-1 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK293-T, HepG2, Jurkat, and MCF-7 cell lysates. Flow Cyt (intra): Jurkat cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5358 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109501于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/1000 - 1/10000. Predicted molecular weight: 149 kDa.
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 149 kDa. |
靶标
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功能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
序列相似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻译后修饰
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
细胞定位
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
别名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
图片
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : PLCG1 knockout HEK-293T cell lysate
Lysates/proteins at 40 µg/ml per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution
Predicted band size: 149 kDaLanes 1- 2: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab109501 was shown to react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type HEK-293T and PLCG1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.cn/ps/products/109/ab109501/Images/ab109501-259086-ab109501KOWB.jpg)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Phospholipase C gamma 1 / PLC-gamma-1 knockout HAP1 cell lysate (20 µg)
Lane 3: HepG2 cell lysate (20 µg)
Lane 4: Mouse brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109501 was shown to recognize Phospholipase C gamma 1 / PLC-gamma-1 when Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Phospholipase C gamma 1 / PLC-gamma-1 knockout samples were subjected to SDS-PAGE. ab109501 at a dilution of 1/1000 and ab8245 (loading control to GAPDH) at a dilution of 1/10000 were incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.cn/ps/products/109/ab109501/Images/Phospholipase-C-gamma-1-Primary-antibodies-ab109501-2.jpg)
Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 149 kDa -
![Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.cn/ps/products/109/ab109501/Images/Phospholipase-C-gamma-1-Primary-antibodies-ab109501-4.jpg)
Flow Cytometry (Intracellular) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab109501 at 1/10 dilution (red)or a rabbit IgG (negative) (green).
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![Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)](https://www.abcam.cn/ps/products/109/ab109501/Images/ab109501-5-benefits-of-recombinant-antibodies.png)
Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (ab109501)
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab109501 被引用在 1 文献中.
- Luo J et al. LTB4 Promotes Acute Lung Injury via Upregulating the PLCe-1/TLR4/NF-?B Pathway in One-Lung Ventilation. Dis Markers 2022:1839341 (2022). PubMed: 35059042