Anti-Phospholipase C gamma 1/PLC-gamma-1抗体(ab52200)
Key features and details
- Rabbit polyclonal to Phospholipase C gamma 1/PLC-gamma-1
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-Phospholipase C gamma 1/PLC-gamma-1抗体
参阅全部 Phospholipase C gamma 1/PLC-gamma-1 一抗 -
描述
兔多克隆抗体to Phospholipase C gamma 1/PLC-gamma-1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, African green monkey
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Phospholipase C gamma 1/PLC-gamma-1.
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阳性对照
- Human breast carcinoma tissue (IHC-P).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52200于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa).
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
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WB
1/500 - 1/1000. Detects a band of approximately 149 kDa (predicted molecular weight: 149 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Plays a role in actin reorganization and cell migration. The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. Major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. -
序列相似性
Contains 1 C2 domain.
Contains 1 EF-hand domain.
Contains 2 PH domains.
Contains 1 PI-PLC X-box domain.
Contains 1 PI-PLC Y-box domain.
Contains 2 SH2 domains.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with CLNK (By similarity). The SH3 domain also mediates interaction with RALGPS1. -
翻译后修饰
The receptor-mediated activation of PLC-gamma-1 and PLC-gamma-2 involves their phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. May be dephosphorylated by PTPRJ.
Ubiquitinated by CBLB in activated T-cells. -
细胞定位
Cell projection > lamellipodium. Cell projection > ruffle. Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment. - Information by UniProt
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数据库链接
- Entrez Gene: 5335 Human
- Entrez Gene: 18803 Mouse
- Entrez Gene: 25738 Rat
- Omim: 172420 Human
- SwissProt: P19174 Human
- SwissProt: Q62077 Mouse
- SwissProt: P10686 Rat
- Unigene: 268177 Human
see all -
别名
- 1 phosphatidyl D myo inositol 4 5 bisphosphate antibody
- 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 1 antibody
- 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200)
Paraffin-embedded human breast carcinoma tissue stained for Phospholipase C gamma 1/PLC-gamma-1, using ab52200 (1/100). The right hand panel represents a negative control where ab52200 was pre-incubated with the immunizing (blocking) peptide.
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Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200)All lanes : Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody (ab52200) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min).
Lane 2 : Extracts from COS7 cells treated
with EGF (200ng/ml, 30min). For this panel, ab52200 was pre-icubated with the immunizing (blocking) peptide.
Predicted band size: 149 kDa
Observed band size: 149 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab52200 被引用在 1 文献中.
- Filhol O et al. CK2β Is a Gatekeeper of Focal Adhesions Regulating Cell Spreading. Front Mol Biosci 9:900947 (2022). PubMed: 35847979