Anti-Phospholamban (phospho S16)抗体(ab15000)

Thank you for contacting us. We have not tested these antibodies in guinea pig and therefore cannot guarantee they will work, however there is high homology between the immunogen and the guinea pig sequence for all three. Normally we predictlikely reactivity if there is 85% homology of the immunogen with the species, and for ab15000 there is 91%, ab85146 is 90% and ab86930 is 93%. The two phospholambin antibodies ab85146 and ab86930 are very similar. They are both rabbit polyclonal purified antibodies and have similar likelihood of reactiving with guinea pig. They do have difference concentrations and volumes (ab85146 is provided at 1ug/ml and 100 ul; ab86930 is 0.5 ug/ml and 200 ul) and have different immunogens. Either would be a good choice.


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Thank you for contacting Abcam.

I would be very happy to help answer your questions:

1 - What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig?

- Both antibodies target different areas of the Phospholamban protein. For a lot of targets, Abcam offers multiply antibodies, that either target different domains, or are more suited to different applications, depending on the customers’ needs.

I cannot find the guinea pig sequence for Phospholamban, therefore I cannot make a recommendation as to whether either of these antibodies would work for that species. If you could please supply the guinea pig sequence, I would be happy to do the alignment and see if there is a chance that either will work for you.

2 - Are there other antibodies more suitable for Phospholamban and Phospholamban(phospho S16) on guinea pig?

- Again, without the guinea pig sequence, I cannot confidently answer this question.

3 - Which lysate solution is best for Phospholamban and Phospholamban(phospho S16)?

- From the information available on the webpages, it looks as though mouse, rat, human and bovine cardiac tissue either treated with or without PKA would be suitable lysates.

4 - Which protease and phosphatase inhibitors are best for the two protein?

- This would be determined on your exact experiment at hand, below is a link to some protease inhibitors, however if you require phospahatse inhibitors then, we unfortunately do not carry those in our catalogue:

https://www.abcam.com/Protease-Inhibitor-Cocktail-ab65621.html



5. Would you like give the protocol for the phosphorylation protein,such as phospho S16?

- For ab15000 was treated with PKA to induce phosphorylation.

Please let me know if there is anything else I can help you with, or if you would like to me to perform the alignment between the guinea pig sequence and the immunogen sequences for these antibodies.

I am sorry to hear that you have been experiencing problems using this product in the application that you wish. Unfortunately, the attachment has not come through successfully. Could you please resend it to me? It is much appreciated.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Please could you provide some further details of the protocol used and complete the following form (attached as a word document).

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.